不同肿瘤标志物在子宫不典型平滑肌瘤组织中的表达及对预后的评估价值

The expressions of PR,p16,p53 and PHH3 in atypical leiomyoma of uterus tissues and their diagnostic value

目的研究PR、p16、p53、PHH3在子宫不典型平滑肌瘤组织中的表达情况,探讨其诊断价值及患者预后的相关性,指导临床。方法选取2014年9月至2016年1月妇产科手术切除的子宫不典型平滑肌瘤56例,收集资料进行随访,并且利用免疫组化的方式检测PR、p16、p53、PHH3在患者体内的水平做出分析。结果 56例子宫不典型平滑肌瘤患者中复发15例,复发率26.79%,对是否复发患者蛋白表达情况的统计,p16在复发患者中阳性率为86.67%,在未复发患者中的阳性率为46.34%,差异有统计学意义(P<0.05),其他蛋白标志物比较差异有统计学意义(P<0.05)。根据肿瘤单发或多发进行分类,其中肿瘤单发15例,占62.5%,对各种蛋白水平进行统计,单发患者中PHH3水平阳性率为94.29%,多发PHH3的阳性率为57.14%,差异有统计学意义(P<0.05),其他蛋白标志物比较,差异无统计学意义(P>0.05)。根据肿瘤大小进行分类,其中直径>5 cm的占总数的82.14%,比较各种蛋白标志物在不同大小肿瘤中的表达可以看出,四种蛋白质表达差异无统计学意义(P>0.05)。统计患者月经情况,其中49例患者未绝经,占总数的87.5%,比较各种蛋白标志物在已绝经和未绝经女性中的表达情况,绝经患者中p53水平阳性率为100%,未绝经p53的阳性率为51.02%,绝经患者中PHH3水平阳性率为42.86%,未绝经PHH3的阳性率为85.71%,差异有统计学意义(P<0.05),其他蛋白标志物比较,差异无统计学意义(P>0.05)。结论对PR、p16、p53、PHH3进行检测有利于判断患者肿瘤的生长情况及相关预后,但具体机制与实验还需开展进一步研究。

Objective To investigate the expressions of PR,p16,p53 and PHH3 in atypical leiomyoma of uterus tissues,and to explore their diagnostic value and correlation with patient’s prognosis.Methods The data about 56 patients with atypical leiomyoma of uterus who received surgical treatment in our hospital from September 2014 to January 2016 were retrospectively analyzed,moreover,the expression levels of PR,p16,p53 and PHH3 in atypical leiomyoma of uterus tissues were detected by immunohistochemisty and statistically analyzed.Results Among 56 patients with atypical leiomyoma of uterus,there were 15 cases of relapse,with relapse rate being 26.79%,moreover,the positive rate of p16 was 86.67%in patients with relapse,which was 46.34%in patients with relapse after operation,there was significant difference between them(P<0.05),moreover,there were significant difference in the other protein markers between them(P<0.05).According to single or multiple tumor classification,there were 15 cases(62.5%)of single tumor,and the positive rate of PHH3 in patients with single tumor was 94.29%,however,which in patients with multiple tumors was 57.14%,there was significant difference between them(P<0.05).However,there were no significant differences in the other protein markers between them(P<0.05).According to the tumor size,the ones with diameter>5cm accounted for 82.14%,but there were no significant differences in the expression levels of four kinds of protein among different sise of tumors(P<0.05).According to the menstruation state of patients,49 patients had no menopause,which accounted for 87.5%.The positive rate of p53 in patients with menopause was 100%,however,which in patients without menopause was 51.02%,moreover,the positive rate of PHH3 in patients with menopause was 42.86%,however,which in patients without menopause was 85.71%,there were significant differences between them(P<0.05).However there were no significant differences in the other protein markers between them(P<0.05).Conclusion The detection of PR,p16,p53 and PHH3 is beneficial to evaluate the growth state of tumor and prognoses of patients,but their action mechanisms need still to be researched further.