烟草Hsp70基因家族的鉴定及NtHsp70Chl基因的表达分析

Bioinformatics of Tobacco (Nicotiana tabacum) Hsp70 Gene Family and Expression Analysis of NtHsp70Chl in Midrib

为探明烟草热激蛋白70(Hsp70)基因家族的特征及烟叶主脉中NtHsp70Chl基因在烘烤条件下的表达模式,对Hsp70基因家族进行了亚细胞定位、系统进化、基因结构、染色体定位,并对NtHsp70Chl基因进行了烘烤条件下实时荧光定量表达PCR分析。结果表明,烟草基因组中Hsp70基因家族共有61个成员,蛋白序列长度不等,等电点在4.52~9.75范围内,各成员蛋白分别定位于细胞质、内质网、细胞外基质、叶绿体和线粒体中;烟草Hsp70基因家族分为6组,定位于叶绿体的成员均存在Ⅵ-a亚组;61个NtHsp70基因分布于18条染色体上,经亚细胞定位于叶绿体的基因分别位于6、17和19号染色体上;烟草Hsp70家族中具有10个基因重复和5个旁系同源基因;经RT-q PCR检测分析,定位于烟草叶绿体中的NtHsp70Chl基因对高温诱导的敏感性较弱。本研究为深入研究烟草Hsp70的功能奠定了基础。

In order to study the relevant biological information of the tobacco heat shock protein 70(Hsp70)gene family and the specific expression of the chloroplast-localized members NtHsp70Chl in tobacco midrib,subcellular location,system evolution,Gene structure,chromosome location,and real-time fluorescence quantitative expression PCR analysis were carried out.The results showed that 61 members of the tobacco Hsp70 gene family were identified.The numbers of amino acid contained in the protein sequence were different,and the isoelectric point was in the range of 4.52~9.75.And the protein members were predicted to be localized in the cytoplasm,endoplasmic Reticulum,extracellular matrix,chloroplast(4 members)and mitochondria respectively.The tobacco Hsp70 gene family was divided into 6 groups,and the NtHsp70Chl genes were all in the VI-a subgroup.61 NtHsp70 genes are located on 18 chromosomes,and NtHsp70Chl genes were located on chromosomes 6,17,and 19,respectively.In tobacco Hsp70 family,there are 10 duplications and 5 paralogous genes.According to RT-qPCR analysis,the NtHsp70Chl genes in tobacco midrib were weakly sensitive to high-temperature stress.This study laid the foundation for further exploration and application of tobacco Hsp70 functions.