利用废弃烟梗发酵生产白僵菌的响应面优化

Optimization of Fermentation Conditions of Beauveria bassiana Using Waste Tobacco Stem as Fermentation Substrate with Response Surface Methodology

为实现废弃烟梗资源化利用,以废弃烟梗为主要发酵底料,白僵菌产孢量为响应值,采用响应面法(Response Surface Methodology,RSM)对烟梗发酵生产白僵菌的条件进行了优化。通过Plackett-Burman(PB)试验筛选出影响废弃烟梗发酵生产白僵菌最显著的因素,设计最陡爬坡试验逼近最佳条件区域,进一步设计Box-Behnken Design(BBD)响应面优化,分析并确定最佳条件。结果表明,当培养时间为9.40 d,蛋白胨含量1.21%,酵母粉含量1.03%时,白僵菌孢子产量预测最优值为1.17×10^(10)个/g,实际孢子产量为1.13×10^(10)个/g,拟合度达到96.58%,白僵菌产孢量显著提高。因此,采用该方法优化得到的最佳发酵条件合理而有效,对烟草生物防治以及烟梗废料的综合利用具有实际应用价值。

In order to realize the resource utilization of waste tobacco stems and improve the application status on waste tobacco stem,using waste tobacco stem as fermentation substrate,and the production of Beauveria bassiana as response value,the fermentation process of Beauveria bassiana was optimized using the response surface methodology(RSM).Through Plackett-Burman(PB)design,the most significant factors affecting the production of Beauveria bassiana using waste tobacco stem as fermentation substrate were identified.Based on the results of the Plackett-Burman(PB)experiment,the steepest ascent experiment was used to approach the optimal region,and the mathematical model of optimization of Box-Behnken Design(BBD)response surface was established to determine the optimal conditions.The results showed that when the incubation time was 9.40 days,concentration of peptone and yeast powder was 1.21%and 1.03%respectively,the prediction spore yield of Beauveria bassiana was 1.17×1010/g,the actual spore yield was 1.13×1010/g,and the fitting degree was 96.58%.Therefore,the optimal fermentation conditions from RSM are reasonable,and have practical application value in microbial prevention and control and the comprehensive utilization of waste tobacco stems.