IL-22BP在活动期炎症性肠病患者肠黏膜中的表达及其意义

Expression and Significance of IL-22BP in Intestinal Mucosa of Patients With Active Inflammatory Bowel Disease

背景:白细胞介素-22结合蛋白(IL-22BP)是IL-22的内源性抑制剂,可抑制IL-22的肠道保护作用,在活动期炎症性肠病(IBD)患者肠黏膜中的表达及其意义尚未明确。目的:探讨IL-22BP在活动期IBD患者肠黏膜中的表达及其意义。方法:纳入2017年1月—2018年1月上海交通大学附属第一人民医院的25例活动期克罗恩病(CD)、36例溃疡性结肠炎(UC)患者,并以30例结肠息肉患者作为对照。评估CD、UC患者的疾病活动度,采用实时荧光定量PCR和免疫组化法分别检测黏膜中IL-22BP mRNA和蛋白表达,并分析IL-22BP蛋白表达与CD、UC疾病活动度的相关性。结果:与相应对照组相比,CD组、UC组肠黏膜中IL-22BP mRNA表达显著升高(CD:3.59±0.83对1.08±0.45,P<0.001;UC:2.19±0.52对1.05±0.34,P<0.001),IL-22BP蛋白表达亦显著升高(CD:6.12±2.30对1.83±1.86,P<0.001;UC:5.58±2.27对2.23±1.77,P<0.001)。CD、UC患者肠黏膜中IL-22BP蛋白表达与疾病活动度均呈显著正相关(r=0.649,P<0.001;r=0.732,P<0.001)。结论:IL-22BP mRNA和蛋白表达在活动期IBD患者肠黏膜中升高,且IL-22BP蛋白表达与IBD疾病活动度呈正相关。

Background:As the endogenous inhibitor of interleukin(IL)-22,IL-22 binding protein(IL-22BP)inhibits the protective effect of IL-22.Expression and significance of IL-22BP in intestinal mucosa of patients with active inflammatory bowel disease(IBD)remain unclear.Aims:To investigate the expression and significance of IL-22BP in intestinal mucosa of patients with active IBD.Methods:A total of 25 Crohn’s disease(CD)and 36 ulcerative colitis(UC)patients from Jan.2017 to Jan.2018 at Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine were enrolled,and 30 colonic polyp patients were served as controls.The disease activity of CD and UC was assessed.Expressions of IL-22BP mRNA and protein in intestinal mucosa were determined by real-time fluorescent quantitative PCR and immunohistochemistry,respectively.Correlation of IL-22BP protein expression with the disease activity of CD,UC was analyzed.Results:Compared with corresponding control group,expressions of IL-22BP mRNA in intestinal mucosa in CD and UC groups were significantly increased(CD:3.59±0.83 vs.1.08±0.45,P<0.001;UC:2.19±0.52 vs.1.05±0.34,P<0.001),and expressions of IL-22BP protein were also significantly increased(CD:6.12±2.30 vs.1.83±1.86,P<0.001;UC:5.58±2.27 vs.2.23±1.77,P<0.001).Expression of IL-22BP protein in intestinal mucosa was positively correlated with disease activity of CD(r=0.649,P<0.001)and UC(r=0.732,P<0.001).Conclusions:Expressions of IL-22BP mRNA and protein are increased in intestinal mucosa of patients with active IBD,and the expression of IL-22BP protein is positively correlated with disease activity of IBD.