基于PTEN的康莱特诱导人胰腺癌细胞凋亡作用

PTEN-based Kanglaite Induces Apoptosis in Human Pancreatic Cancer Cells

目的研究康莱特注射液分别在PTEN正常表达和低表达时对人胰腺癌细胞凋亡的影响,进而评估康莱特注射液对胰腺癌的治疗作用。方法该研究于2016年2月—2017年9月在哈尔滨医科大学公共卫生学院进行,人胰腺癌PANC-1细胞、沉默PTEN表达的胰腺癌PANC-1细胞分别分成康莱特处理组及未处理组,以流式细胞术检验胰腺癌细胞凋亡改变。结果康莱特处理组胰腺癌细胞凋亡率(59.67±13.23)%明显高于对照组(13.26±4.72)%(P<0.01),sh PTEN+康莱特组和sh PTEN组细胞凋亡率(39.85±13.47)%和(27.37±8.62)%也高于对照组(13.26±4.72)(P<0.05)。结论康莱特注射液以调控PTEN表达的方式诱导人胰腺癌细胞凋亡,康莱特注射液是一种治疗胰腺癌的潜力药物。

Objective This paper tries to study the effect of Kanglaite Injection on the apoptosis of human pancreatic cancer cells in normal and low expression of PTEN,and to evaluate the therapeutic effect of Kanglaite injection on pancreatic cancer.Methods This study was carried out in Harbin Medical University School of Public Health from February 2016 to September 2017 Pancreatic cancer PANC-1 cells and PTEN-expressing pancreatic cancer PANC-1 cells were divided into Kanglaite treatment group and untreated group respectively.The apoptosis of pancreatic cancer cells was examined by flow cytometry.Results The apoptosis rate of pancreatic cancer cells in KLT group(59.67±13.23)%was significantly higher than that in control group(13.26±4.72)%(P<0.01).The apoptotic rates of shPTEN+KLT group and shPTEN group(39.85±13.47)%and(27.37±8.62)%was also higher than the control group(13.26±4.72)%(P<0.05).Conclusion Kanglaite injection induces apoptosis of human pancreatic cancer cells by regulating PTEN expression.Kanglaite injection is a potential drug for pancreatic cancer.