蛇尾草质量标准的研究

Quality standard for Pogostemon auricularius

目的建立蛇尾草Pogonostemon auricularius(L.)Hassk.的质量标准。方法对蛇尾草基源、性状、显微特征进行鉴别后,TLC法作定性鉴定,HPLC法测定毛蕊花糖苷含有量,《中国药典》方法测定水分、总灰分、酸不溶性灰分、浸出物含有量。结果 TLC斑点清晰,重复性好。毛蕊花糖苷在0.102~2.04μg范围内呈良好的线性关系(r=0.999 98),平均加样回收率97.99%,RSD 2.02%。14批样品中,水分、总灰分、酸不溶性灰分、浸出物含有量范围分别为6.47%~12.07%、7.59%~12.35%、0.23%~3.55%、13.28%~26.82%。结论该方法稳定可靠,可用于蛇尾草的质量控制。

AIM To establisli the quality standard for Pofonosternof auriciularkus(L.)Hassk..METHODS The original,characteristic and microscopic features of P.auricularins were identified,after which TLC was used for qualitative identification,HPLC was adopted in the content determination of verl)ascoside,and the contents of water,total ash,acid-insoluble ash,extract were determined by Chinese Pharmacopoeia metiiods.RESULTS The clear TLC plots displayed good reproducibility.Verbascoside showed a good linear relationship within the range of 0.102-2.04"g(r=0.999 98)#whose average recovery was 97.99%witli the RSD of 2.02%.In four-teen batches of samples,thecontent ranges of water,total ash,acid-insoluble ash,extract were 6.47%-12.07%,7.59%-12.35%,0.23%-3.55%,13.28%-26.82%,respectively.CONCLUSION This sta-ble and reliable metliod can be used for the quality control of P.auricularius.