益气解毒方对鼻咽癌细胞标志物T淋巴瘤侵袭转移诱导因子1及Ras相关C3肉毒素底物1表达的影响

Effects of Yiqi Jiedu Prescription on Expressions of Nasopharyngeal Carcinoma Cell Markers Tiam1 and Rac1

目的观察益气解毒方对鼻咽癌细胞标志物T淋巴瘤侵袭转移诱导因子1(Tiam1)和Ras相关C3肉毒素底物1(Rac1)表达的影响,探讨其作用机制。方法实验分为对照组、益气解毒方组和阳性药组,分别予生理盐水、益气解毒方药液和5-氟尿嘧啶注射液,干预后置于37℃、5%CO2孵育箱中培养,24 h后采集细胞。免疫组化和Western blot检测细胞Tiam1和Rac1蛋白表达,RT-PCR检测细胞Tiam1和Rac1 mRNA表达。结果与对照组比较,益气解毒方组和阳性药组Tiam1和Rac1的蛋白和mRNA表达明显降低,且益气解毒方组降低更明显(P<0.01)。结论益气解毒方可能通过抑制肿瘤细胞标志物Tiam1和Rac1的表达,达到治疗鼻咽癌的作用。

Objective To investigate the effects of Yiqi Jiedu Prescription on expressions of nasopharyngeal carcinoma cell markers Tiam1 and Rac1;To explore its possible mechanisms.Methods The experiment included blank group,Yiqi Jiedu Prescription group and positive control group,and the three groups were given saline,Yiqi Jiedu Prescription and 5-Fu injection,respectively.After being intervened,the three group were cultured at 37℃in 5%incubator.After 24 hours,the cells were collected.The protein levels of Tiam1 and Rac1 were evaluated by Western blot and immunohistochemistry.The mRNA levels of Tiam1 and Rac1 were determined by real-time PCR.Results Compared with blank group,protein and mRNA expressions of Tiam1 and Rac1 decreased in Yiqi Jiedu Prescription group and positive control group,and Yiqi Jiedu Prescription group showed more significant decrease(P<0.01).Conclusion Yiqi Jiedu Prescription plays a role in the treatment of nasopharyngeal carcinoma by inhibiting the expression of tumor cell markers Tiam1 and Rac1.