摘要
为探究外源硫化氢(Hydrogen sulfide,H_2S)对Cd胁迫下平邑甜茶幼苗根系损伤的影响,及Ca^(2+)在H_2S介导重金属胁迫信号传导中的作用,采用营养液培养法,利用外源Ca^(2+)以及Ca^(2+)螯合剂EGTA、质膜Ca^(2+)通道阻断剂La3+和钙调素拮抗剂CPZ,研究了平邑甜茶幼苗生长、根系细胞死亡、根系活性氧代谢和线粒体特性的变化。结果表明:与单独Cd胁迫处理相比,添加外源Ca^(2+)或H_2S供体硫氢化钠(Na SH)均显著缓解了Cd胁迫对平邑甜茶幼苗生长的抑制,根系细胞死亡数量减少,根系超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性显著提高,超氧阴离子产生速率(O2-·)、过氧化氢(H_2O_2)和丙二醛(MDA)含量明显下降,根系线粒体膜透性(MPT)减小,膜电位(Δψm)提高、细胞色素c(Cyt c)含量增加;添加Na SH的同时添加钙离子专一螯合剂EGTA,钙离子通道阻断剂La Cl3或钙调素拮抗剂CPZ抑制了H_2S以上的调节作用。可见,外源Ca^(2+)或H_2S可稳定线粒体功能,增强活性氧清除能力,减轻Cd对平邑甜茶的伤害,并且在这个过程中H_2S缓解Cd胁迫的作用可能依赖于Ca^(2+)。
The purpose of this paper is to explore the effects of hydrogen sulfide(H 2 S)on root injury of Malus hupehensis Rehd.under Cd stress.The nutrient solution culture was used to study the effects of exogenous Ca 2+,EGTA(Ca 2+chelator),La 3+(blocker of Ca 2+channel in plasma membrane)and CPZ(antagoniston of calmodulin)on the growth of seedling,root cell death,reactive oxygen species metabolism and mitochondrial properties.The results showed that adding exogenous Ca 2+or H 2 S donor NaSH could obviously alleviate the inhibition to the growth of seedlings,reduced the number of cell death in roots induced by Cd stress;and compared with Cd stress alone,the enzymes(SOD,POD,CAT)activities were significantly increased,the rate of superoxide anion production(O 2-[]·),contents of hydrogen peroxide(H 2 O 2)and malondialdehyde(MDA)were significantly decreased,the mitochondrial membrane permeability(MPT)of root was decreased,the mitochondrial membrane potential(Δψm)of root was increased,the content of cytochrome c(Cyt c)was increased.The alleviative effects of H 2 S on cadmium injury were inhibited by addition of Ca 2+-chelate EGTA,Ca 2+channel blocker LaCl 3 or calmodulin inhibitor CPZ.Those indicate that Ca 2+or H 2 S can stabilize mitochondrial function,enhance the ROS scavenging activity,alleviate the injury of Cd to Malus hupehensis Rehd.,and the effect of H 2 S on cadmium stress may be dependent on Ca 2+in this process.
作者
曹慧
潘利
姜倩倩
邹岩梅
束怀瑞
CAO Hui;PAN Li;JIANG Qianqian;ZOU Yanmei;SHU Huairui(Weifang University,Key Laboratory of Biochemistry&Molecular Biology in Universities of Shandong, Weifang 261061,China;Weifang No.1 High School,Weifang 261041,China; 3.National Engineering Research Center for Apple,Tai′an 271018,China)
出处
《华北农学报》
CSCD
北大核心
2018年第2期163-168,共6页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(31301733)
潍坊市科技发展计划项目(20121303)
潍坊学院博士基金项目(2012BS18)
关键词
平邑甜茶
钙
硫化氢
活性氧
线粒体
Malus hupehensis Rehd.
Cadmium
Hydrogen sulfide
Reactive oxygen species
Mitochondria