拮抗植物病原真菌Streptomyces corchorusii AUH-1的分离与鉴定

Isolation and identification of Streptomyces corchorusii AUH-1 with antagonistic activity against plant pathogenic fungi

为了获得对植物病原真菌具有抑制作用的拮抗菌株,以西瓜枯萎病菌为指示菌,采用平板对峙试验进行植物病原真菌拮抗菌的分离筛选。从南昌、赣州、鹰潭、萍乡、武夷山、石家庄等地采集的26个不同类型土样中,分离筛选到1株对西瓜枯萎病菌具有良好抑制作用的菌株AUH-1,其抑菌率达到36.15%。根据菌体形态学观察、生理生化特征以及16S r DNA序列分析,对拮抗菌进行菌种鉴定,结果表明该菌株为黄麻链霉菌(Streptomyces corchorusii),并命名为黄麻链霉菌AUH-1。

In order to screen antagonistic bacterium which could inhibit the growth of phytopathogenic fungi,the antagonistic strain was isolated by screen with Fusarium oxysporum f.sp.niveum as indicator and then using dual culture.An antagonistic bacterium AUH-1 against F.oxysporum f.sp.niveum was isolated from 26 different types of soil samples collected from Nanchang,Ganzhou,Yingtan,Pingxiang,Wuyishan and Shijiazhuang,etc.,with the inhibition rate of 36.15%.Based on morphological characteristics,physiological and biochemical characteristics,and 16S rDNA sequence analysis,the strain was preliminarily identified as Streptomyces corchorusii and named as S.corchorusii AUH-1 here.