广藿香的组培快繁技术研究

Research on the tissue culture and rapid propagation of Patchouli

以广东省遂溪县乌塘镇广藿香嫩茎为外植体,研究影响丛生芽诱导、增殖与壮苗生根的主要因素。结果表明:遂溪乌塘广藿香的外植体处理过程中,用0.1%升汞(加2滴吐温-80)溶液消毒8min.,污染率和褐变率最低;适宜丛生芽诱导分化的培养基是MS+6-BA1.0mg/L+NAA0.2mg/L;最佳增殖培养基是MS+6-BA0.5mg/L+NAA0.2mg/L;生根培养基以MS+IAA0.5 mg/L+IBA0.5 mg/L最佳,生根率达100%,而且根系发育良好。

The fresh stems of GuangDong Suixi Wutang Patchouli was used ex-plant to establish a research on the major factor for the induction and proliferation of clustered shoots and sound seed take root.The study showed that:the Control of the length of time spent on the disinfecting time plays a major role on the success rate of ex-plant induction during the process of Wutang Patchouli ex-plant;the suitable medium for the induction and differentiation of clustered shoots is MS+6-BA1.0mg/L+NAA0.2mg/L;the best proliferation medium is MS+6-BA0.5mg/L+NAA0.2mg/L;the rooting medium of MS+IAA0.5 mg/L+IBA0.5 mg/L is the best,which has a 100%rooting percentage,and the root development is good.