气相色谱法检测再造烟叶浓缩液浓度

Determination of concentration of concentrated solution in reconstituted tobacco manufacturing process by gas chromatography

为准确测定再造烟叶浓缩液浓度,对萃取剂、萃取时间、称样量等处理条件进行了优化,建立了测定再造烟叶浓缩液浓度的气相色谱法。1)优化的检测条件为:采用100 mL含内标物异丙醇的甲醇为萃取剂对1 g再造烟叶浓缩液样品进行振荡萃取10 min;2)实验的范围内,测定结果重复性好,RSD为0.572%~0.681%,再现性高,RSD为0.694%~1.227%,加标回收率为100.3%~101.7%,检测限为0.0144%,定量限为0.048%;3)与烘箱法相比,本方法能消除浓缩液中易挥发组分高温下散失而对结果的影响,结果更为准确。

In order to determine accurate concentration of reconstituted tobacco concentrate,gas chromatography based method was developed.Treatment conditions including extraction solvent,extraction time and sample weight were optimized.Results showed that:1)the optimal determination results were achieved when 1 g of sample was extracted under shaking for 10 min using 100 mL of methanol as extraction solvent and isopropanol as internal standard.2)The variation coefficient of repeat test was ranged from 0.572 to 0.681%,the variation coefficient of reproducibility test varied from 0.694 to 1.227%,the recoveries range was from 100.3 to 101.7%,the detection limit was 0.0144%,the quantification limit was 0.048%.3)Compared with oven drying method,this method can achieve a more accurate result by eliminating errors due to volatilization of volatile components under high temperature.