糖尿病视网膜病变患者高密度脂蛋白血管保护功能的研究

Vascular protection dysfunction of high-density lipoprotein in patients with diabetic retinopathy

目的探讨糖尿病视网膜病变(diabetic retinopathy,DR)患者体内高密度脂蛋白high-density lipoprotein,HDL)的血管保护功能是否发生异常。方法选取DR患者23例DR组)和正常对照21人(正常对照组),提取所有研究对象血浆中的HDL。分别使用2组获得的HDL和相同体积PBS(空白对照组)处理人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC),行细胞增殖实验和细胞迁移实验观察3组HUVEC迁移能力和增殖能力的变化。结果与空白对照组相比,正常对照组的HDL可以明显促进HUVEC增殖和迁移;与正常对照组HDL相比,DR组的HDL促进HUVEC增殖和迁移的能力明显下降,其中促细胞增殖能力下降约14.8%(P=0.027 1),促细胞迁移能力下降约49.3%(P=0.009 6)。结论 DR患者HDL促进HUVEC增殖和迁移的能力明显下降,即其血管保护功能发生了异常。

Objective To explore changes in the vascular-protecting function of HDL in patients with diabetic retinopathy(DR).Methods Together 23 patients with diabetic retinopathy(DR,DR group)and 21 normal subjects(normal control group)were recruited,and the 2 mL blood were collected from each of these subjects for isolating high-density lipoprotein(HDL).And then human umbilical vein endothelial cells(HUVECs)treated with harvested HDL and PBS(blank control group)were used for detection of cell proliferation and migration in the three groups.Results Compared with the blank control group,HDL in the normal control can significantly promote the proliferation and migration of HUVECs;but when compared with the normal control group,the ability of HDL to promote cell proliferation and migration significantly decreased in the DR group,in which the proliferative capacity of the cells decreased by approximately 14.8%(P=0.027 1),and the migration ability decreased by about 49.3%(P=0.009 6).Conclusion HDL from DR patients is dysfunctional in stimulating endothelial cell migration and proliferation,which means that vascular-protecting function of HDL in patients with DR is abnormal.