Hippo信号通道中Lats2基因表达与湖羊肌肉生长发育的关系

Relationship between Lats2 gene in Hippo signaling pathway and Hu sheep muscles growth traits

[目的]探究Hippo信号通道中Lats2基因与湖羊肌肉生长发育的关联性。[方法]以湖羊作为试验材料,用RT-qPCR技术分析Lats2基因在不同性别、不同生长阶段(2、60、180日龄)、不同肌肉组织(比目鱼肌、背最长肌、腓肠肌和趾长伸肌)的时空表达规律,及其与Lats1、YAP1(Yes相关蛋白1)基因和肌肉生长相关基因在不同生长阶段的相关性。[结果]根据Hippo信号通道中Lats2基因的时空表达规律可以发现:在不同肌肉组织中,Lats2基因在趾长伸肌中相对表达量最高;在不同生长阶段,Lats2基因在60日龄的表达量最高;在不同性别中,Lats2基因的表达表现为公羊高于母羊。相关性分析结果表明:在2日龄肌肉组织中,Lats2基因的表达与MSTN基因间正相关(P<0.05);在60日龄的肌肉组织中,Lats2基因的表达与Lats1基因正相关(P<0.05);在180日龄肌肉组织中,Lats2基因的表达与MyoG基因间极显著正相关(P<0.01),与YAP1基因间显著负相关(P<0.05)。[结论]Hippo信号通道中Lats2基因的表达受不同性别、年龄和肌肉组织的影响,可能通过下调YAP1基因的表达抑制肌肉生长发育,可作为肌肉生长发育调控研究的候选基因。

[Objectives]The experiment mainly explored the correlation between large tumor suppressor kinase 2 gene(Lats 2)in Hippo signal channel and Hu sheep muscle growth by RT-qPCR technique.[Methods]In this study,we analyzed the spatial and temporal expression regulation of Lats 2 in Hu sheep with the affection of different gender,growth stages(2,60 and 180 day-old)and skeletal muscle tissue(soleus muscle,longissimus doris muscle,gastrocnemius muscle,extensor digitorum longus).The correlation of expression in different growth stages within large tumor suppressor kinase 1 gene(Lats 1),Yes-associated protein 1 gene(YAP 1)and genes,which could affect the growth of muscle tissue(MyoG,MyHCⅠ,MSTN,MyHCⅡA,MyHCⅡX),or the genes related to muscle growth was emphatically discussed.[Results]It suggested that Lats 2 in Hippo signal channel had the highest related expression in the extensor digitorumlongus muscle on the analysis of different muscle tissue expression.Affected by different growth stages,Lats 2 gene had the highest related expression in the 6-month-old,and higher in the 2-day-old and the 2-month-old.In different genders,the related expression of rams was higher than that of ewes.On the analysis of related expression of Lats 2,Lats1,YAP 1 and genes that could affect the growth of muscle tissue,we found that Lats 2 in 2-day-old muscle tissue had significant positive correlation with MSTN(P<0.05).In 2-day-old muscle tissue,there was a significant positive correlation between Lats 2 and Lats 1(P<0.05).The expression of Lats 2 and MyoG had highly significant positive correlation(P<0.01),and the correlation of the expression between Lats 2 and YAP 1 was significant negative in 6-month-old muscle tissue(P<0.05).[Conclusions]The expression of Lats 2 gene in Hippo signal channel was affected by different gender,growth stages and skeletal muscle tissue.We can down-regulate the expression of YAPA1 to inhibit the muscle growth,which can be a candidate gene of the growth of muscle fiber.