抑制性消减杂交分析肌动蛋白细胞骨架和细胞外基质基因在膀胱癌中的表达下调

Identification of actin cytoskeleton and extracellular matrix down-regulation in human bladder cancer by suppression subtractive hybridization

目的 分析膀胱移行细胞癌的差异表达基因 ,探讨肿瘤行为的分子生物学基础及筛选有效的肿瘤标记。方法 应用抑制性消减杂交 (SSH)方法构建在正常膀胱组织和膀胱癌组织中差异表达序列的消减文库 ,用差异筛选技术分离差异表达基因。结果 从消减文库中共获得了 9个在正常膀胱组织中高表达、在膀胱癌组织中表达下调的基因 ,分别属于细胞外基质成分和肌动蛋白细胞骨架体系。结论 细胞外基质和肌动蛋白细胞骨架体系中相关基因的表达下调可能反映了肿瘤细胞在细胞粘附、细胞间相互作用、迁移及运动等诸多过程中的异常 ,可能与癌细胞的侵袭和转移密切相关 ,对发展成为膀胱癌的分子标记物具有潜在价值 ;gelsolin。

Objective To identify the genes differentially expressed in human urinary transitional cell carcinoma.Methods Suppression subtractive hybridization was performed using primary tumor tissues as driver and normal urinary epithelia as tester.Down regulated genes in tumor cells were identified by differential screening method.Results 9 genes were identified in bladder tumor tissues showing decreased mRNA levels.They either belong to the extracellular matrix family.Conclusion Alterations of extracellular matrix components and actin cytoskeleton may be general effector events during the development of bladder cancer and may provide potential tumor biomarkers.Gelsolin and tropomyosin may play tumor suppressor roles in the tumorigenesis of bladder cancer.