SCoT分子标记对不同产地白芍亲缘关系的研究

Study on the Genetic Relationship of Paeonia lactiflora from Different Regions by SCoT Molecular Markers

目的:本研究采用SCoT分子标记技术对分布于浙江磐安,安徽毫州,四川中江,山东菏泽共22个居群的白芍样品进行亲缘关系的研究。方法:通过提取白芍DNA,正交设计寻找白芍SCoT分子标记最佳PCR反应体系,筛选获得白芍PCR扩增的SCoT引物,应用NTSYS2.10e软件并采用UPGMA法进行聚类分析。结果:发现SCoT引物碱基序列共扩增出121条带,其中多态性条带有77条,多态百分率为63.6%,各品种间的相似系数在0.78~0.94之间,不同产地白芍分别聚为一类。结论:不同产地白芍有着丰富的遗传多态性,且SCoT分子标记适用于不同产地白芍亲缘关系的研究,有效区分不同产地白芍品种,这为白芍药材种质资源的引种和规范栽培提供了有力参考。

Objective:This study used SCoT molecular marker technique to find the relationship between the 22 groups of Paeoniae lactiflora samples,which were distributed in the Zhejiang panan,Anhui haozhou,Sichuan zhongjiang and Shandong heze.Methods:Extracted Paeonia lactiflora DNA,Optimal PCR reaction system and found the best suitable for SCoT molecular marker of P.lactiflora by orthogonal design.Screened the SCoT primers obtained from PCR amplification.Finally,applied the NTSYS2.10e software and the UPGMA method was used for cluster analysis.Results:Finded that SCoT primer sequence amplificated out 121 bands,included 77 polymorphism bands,polymorphic percentage was 63.6%,the varieties of similarity coefficient between 0.78~0.94,P.lactiflora of different regions were respectively divided into category.Conclusion:The results showed that there was a rich genetic polymorphism of P.lactiflora in different habitats,and the SCoT molecular marker was suitable for the study of the relationship between P.lactiflora from different sources.The effective differentiation of P.lactiflora varieties from different habitats provided a strong reference for the cultivation and standardization of germplasm resources of P.lactiflora