利用网络药理学方法预测金丝桃苷治疗动脉粥样硬化的作用靶点及作用机制

Prediction of Target and Mechanism of Hyperoside in Atherosclerosis Treatment by Using Network Pharmacology Methods

目的:采用网络药理学的方法对金丝桃苷治疗动脉粥样硬化的作用靶点进行预测,探讨其可能的作用机制。方法:通过TCMSP数据库和GAD数据库分别检索金丝桃苷和动脉粥样硬化的作用靶点,将得到靶点信息导入Cytoscape-v3.2.1软件,构建金丝桃苷靶点蛋白质相互作用关系PPI网络和动脉粥样硬化靶点蛋白质相互作用关系PPI网络,取两个PPI网络的交集,得到交集节点蛋白质,经过筛选得到核心靶点蛋白质,利用DAVID数据库对核心靶点蛋白质进行GO注释分析和KEGG通路分析。结果:EGFR、GRB2、NTRK、HSP90AA1、HSP90AB1、FBO6、PSMA3、PTPN1、HUWE1和VCP是金丝桃苷治疗动脉粥样硬化的10个核心靶点,分别在29个(P<0.05)和12个(P<0.01)生物过程、7条(P<0.05)和4条(P<0.01)信号通路中有富集,其中PI3K/AKT和MAPK两条信号通路在GO注释分析和KEGG通路分析中都有富集。结论:金丝桃苷治疗抗动脉粥样硬化的作用机制可能主要与PI3K/AKT和MAPK两条信号通路有关。

Objective:To Study the effect of hyperoside in the atherosclerosis treatment by network pharmacology,and explore its possible mechanism.Methods:The target information was introduced into Cytoscape-v3.2.1 software by using the TCMSP database and the GAD database to find the target of hyperoside and atherosclerosis.The target of hyperoside and atherosclerosis protein interaction PPI network were constructed.Taking two PPI network intersections,the intersection node protein was obtained.The core target protein was obtained after screening.The DAVID database was used for the core target protein GO comment analysis and KEGG pathway analysis.Results:EGFR,GRB2,NTRK,HSP90AA1,HSP90AB1,FBO6,PSMA3,PTPN1,HUWE1 and VCP were the 10 core targets of hyperoside in atherosclerosis treatment in 29(P<0.05)and 12(P<0.01),and 7(P<0.05)and 4(P<0.01)signaling pathways,among them the PI3K/AKT and MAPK signaling pathways enriched in GO annotation analysis and KEGG pathway analysis.Conclusion:The mechanism of hyperoside treatment against atherosclerosis may related to PI3K/AKT and MAPK signaling pathways.