桃花中绿原酸抗氧化活性的研究

Antioxidant Activity of Chlorogenic Acid Extracted from Peach Blossom

以桃花提取物为试材,维生素C和绿原酸标准品为对照品,采用K_3[Fe(CN)_6]测定其还原能力,D-脱氧核糖-铁体系法、二苯代苦味酰基自由基(DPPH·)和邻苯三酚自氧化法测定桃花提取物中绿原酸的抗氧化活性。结果表明:桃花提取物具有较强的还原性,其抗氧化活性与绿原酸含量密切相关。其中桃花提取物抗羟基自由基活性高于Vc,浓度为200μ·mL^(-1)时,可达到66.42%;抗超氧阴离子自由基活性略低于Vc,浓度为200μg·mL^(-1)时,抗氧化活性为95.78%;抗DPPH·自由基的活性比Vc略高,当浓度为200μg·mL^(-1)时,抗氧化活性为82.12%。该研究说明,桃花提取物的还原性和抗氧化活性与绿原酸具有良好的效量关系,桃花中的抗氧化活性物质主要是绿原酸。

Taking Peach blossom as test material,antioxidant activity of chlorgenic acid extracted from peach blossom was measured by D-deoxyribose-iron system method along with 2,2-diphenyl-1-picrylhydrazyl pyrogallol autoxidation method,while vitamin C and chlorgenic acid were used as reference substance and reduction ability of the extract was measured by using K3\[Fe(CN)6\].The results showed that peach blossom extract had strong reducibility,and its antioxidant activity was closely related to chlorgenic acid content.The extract had higher anti-hydroxyl and anti-DPPHo activity but lower anti-superoxide anion activity than vitamin C.Its reducibility with concentration of 200μg·mL-1 for hydroxyl was as high as 66.42%,and antioxidant activity was 95.78%and 82.12%for superoxide anion and DPPHo,respectively.This study showed that the main extract of peach blossom was chlorgenic acid and its concentration largely determine reductive and antioxidant activities for the extract.