大鼠膀胱癌模型的光动力学治疗

Efficacy of photodynamic therapy on rat bladder cancer model

目的 验证采用新型光敏剂的光动力学治疗方法对于SD大鼠膀胱癌动物模型的有效性。方法 使用 0 .0 5 %N 丁基 N(4 羟丁基 )亚硝胺 (BBN)诱导成功的SD大鼠随机分为试验组2 0只和对照组 7只。实验组大鼠用剂量为 50mg/kg体重光敏剂溶液灌胃 ,2 4h后打开膀胱进行激光照射 ,能量为 50J/cm2 ,72h后取肿瘤标本送病理分析和DNA含量测定 ,对照组术前不灌药物。结果 光动力学治疗法 (PDT)治疗后试验组的肿瘤坏死发生率和面积均高于对照组 ,差异有显著性 (P <0 .0 5)。样本的平均DNA质量均数 :实验组 9.50 5pg,对照组 1 4 .2 69pg,差异有显著性 (P<0 .0 5)。结论 以CDHS80

Objective To estimate the efficacy of photodynamic therapy in which a new drug CDHS801 acts as the photosensitizer on established orthotopic SD rat bladder cancer model.Methods An orthotopic SD rat bladder cancer model induced by 0.05% N butyl N (4 hydroxybutyl) nitrosamine was established at the beginning of the study.27 rats were randomly divided into experiental group(n=20) and contral group(n=7).CDHS801 was administrated orally in experimental group 24 h before PDT operation which was performed under general anesthesia.In the PDT operation,a λ=650?nm semiconductor laser equipment was used.The bladder of rats was opened and an optic fiber tip was aimed onto the tumor with radiation dosage of 50?J/cm 2.72 h later,biopsy was performed on all the rats and the pathological samples were collected for comparison in terms of the size of tumor necrosis area and DNA mass.Results After PDT,a significant higher amount of necrosis and some kind of necrosis presentation were observed in the experimental group than in the control group (P<0.05).The incidence of necrosis was also higher in the experimental group (P<0.05).The mean DNA mass also showed significant difference between experimental group and contral group (P<0.05).Conclusion CDHS801 assisted photodynamic therapy is an effective alternative for the transitional bladder tumor of rats.