复方鳖甲软肝片对二乙基亚硝胺所致原发性肝癌大鼠肝组织PCNA和α-SMA表达的影响

Protective effect of Fufangbiejia, a herbal medicine, on diethylnitrosamine-induced primary liver cancer in rats and its impact on PCNA and α-SMA expression

目的研究复方鳖甲软肝片对二乙基亚硝胺(DEN)所致原发性肝癌大鼠肝癌组织增殖细胞核抗原(PCNA)和平滑肌肌动蛋白(α-SMA)表达的影响。方法将40只SD大鼠随机平均分为对照组、模型组、大剂量和小剂量复方鳖甲软肝片处理组,每组均为10只。应用DEN注射法制备原发性肝癌模型,分别给予复方鳖甲软肝片0.625 g.kg^(-1)和1.25 g.kg^(-1)或生理盐水灌胃,连续给药20周。采用免疫组化法检测肝组织PCNA和α-SMA表达。结果大剂量组肝脏质量、肝脏癌变率、肝脏指数和肝表面癌结节数分别为(17.34±1.78)g、10.0%、(3.10±0.72)和(3.16±0.45)个,显著低于模型组【(25.59±2.38)g、100.0%、(4.95±0.58)和(14.14±2.37)个,P<0.05】;小剂量组肝脏质量、肝脏癌变率、肝脏指数和肝表面癌结节数分别为(19.89±1.80)g、30.0%、(3.89±0.81)和(7.38±1.01)个,也显著低于模型组(P<0.05);大剂量组PCNA和α-SMA相对表达量分别为(5.98±1.12)和(7.96±1.37),小剂量组分别为(9.23±1.34)和(12.34±2.88),均分别明显低于模型组【(19.59±2.67)和(25.12±3.19),P<0.05】。结论复方鳖甲软肝片对DEN所致原发性肝癌大鼠具有较好的防治作用,可能是通过调节肝组织PCNA和α-SMA表达发挥作用的。

Objective To observe the protective effect of Fufangbiejia,a herbal medicine,on diethylnitrosamine(DEN)-induced primary liver cancer(PLC)in rats and its impact on proliferating cell nuclear antigen(PCNA)andα-SMA expression.Methods 40 SD rats were randomly divided into control,model,high and low dose of herbal medicine-intervened groups,with 10 in each.The DEN was used to induce hepatic neoplasmas in rats,and the herbal medicine at dose of 0.625 g·kg-1 and 1.25 g·kg-1 or saline for gavage were given for 20 weeks.The PCNA andα-SMA were stained immunohistochemically.Results The liver weights,surface carcinoma nodule numbers,canceralization rates and liver index were(17.34±1.78)g,10.0%,(3.10±0.72)and(3.16±0.45)in high dose group,significantly lower than in the model group【(25.59±2.38)g,100.0%,(4.95±0.58)and(14.14±2.37),P<0.05】;the liver weights,surface carcinoma nodule numbers,canceralization rates and liver index were(19.89±1.80)g,30.0%,(3.89±0.81)and(7.38±1.01)in low dose group,also significantly lower than in the model group and(P<0.05);the expression scores of PCNA andα-SMA were(5.98±1.12)and(7.96±1.37)in high dose group,and(9.23±1.34)and(12.34±2.88)in low dose group,both significantly lower than in the model group【(19.59±2.67)and(25.12±3.19),P<0.05】.Conclusions Fufangbiejia has a protective effect on DEN-induced primary liver cancer in rats,which might be related to the regulation of hepatic PCNA andα-SMA expression.