丙肝病毒核心蛋白对肝门部胆管癌细胞增殖和细胞凋亡的调控作用

Hepatitis C virus core protein modulating proliferation and apoptosis of hilar cholangiocarcinoma tissues

目的 探讨丙肝病毒核心蛋白 (HCVC蛋白 )在肝门部胆管癌发生和发展中的作用。方法 采用过氧化物酶 抗过氧化酶 (PAP)法检测 36例肝门部胆管癌组织中HCVC蛋白的表达 ,原位末端标记技术 (ISEL)和链霉亲合素 生物素技术 (SLAB)分别检测肝门部胆管癌中的细胞凋亡指数 (AI)和细胞增殖指数 (PI)。结果 36例肝门部胆管癌患者中HCVC蛋白阳性表达率为61 .1 % (2 2 /36) ,AI和PI的平均值分别为 (3 .46± 0 .62 ) %和 (46 .43± 1 2 .59) % ,HCVC蛋白阳性表达组中AI明显低于HCVC蛋白阴性表达组 (P <0 .0 1 ) ,而PI在HCVC蛋白阳性组中明显高于HCVC蛋白阴性组 (P <0 .0 1 )。结论 HCVC蛋白可能有助于肝门部胆管癌细胞增殖 。

Objective To explore the role of hepatitis C virus core protein in the development of hilar cholangiocarcinoma tissues.Methods Thirty cases of surgical specimens from hilar cholangiocarcinoma were studied by immunohistochemical staining for hepatitis C virus core protein (HCV C protein) and PCNA index (PI). In situ end labeling and immunohistological SLAB methods were used to detect aopototic index (AI) and PI in hilar cholangiocarcinoma.Results The positive expression of HCV C protein in the patients with hilar cholangiocarcinoma was 61.6% (22/36). The mean values of AI and PI were (3.46±0.62)% and (46.43±12.59)% respectively. PI in hilar cholangiocarcinoma tissues positive for HCV C protein was significantly higher than those negative for HCV antigen (P<0.01),while AI in the hilar cholangiocarcinoma tissues positive for HCV C protein was significantly lower than those negative for HCV C protein (P<0.01).Conclusion HVC C protein may contribute to hilar cholangiocarcinoma proliferation and reduce apoptosis of hilar cholangiocarcinoma.