用改进石蜡切片法观察大鳞副泥鳅皮肤虹彩细胞

Improved paraffin section method for observing iridophores in skin of Paramisgurnus dabryanus

以大鳞副泥鳅为研究对象,针对常规石蜡切片无法观察到鱼类皮肤虹彩细胞的不足,固定液改用10%中性福尔马林,脱水前使用1.5%琼脂糖对组织进行预处理,染色使用3%伊红。结果发现:大鳞副泥鳅新鲜皮肤组织选用10%中性福尔马林进行固定,在梯度脱水前用1.5%琼脂糖对组织进行预处理,并用3%伊红进行染色,即可以得到组织结构完整、虹彩细胞清晰的皮肤组织切片。表明改进后的石蜡切片法可以用于鱼类皮肤虹彩细胞观察。

The iridophores in the fish skin could not be observed by traditional paraffin section method,so Paramisgurnus dabryanus was used to improve the method in this experiment.The fixative was changed to 10%neutral formalin.Moreover,1.5%agarose was used to pretreat the skin before dehydration,and 3%eosin was used to stain the skin.The results showed that fixing the skin of P.dabryanus by 10%formalin,pretreating by 1.5%agarose before dehydration and staining with 3%eosin,could get the slices with intact organizational structure and clear iridophores.Therefore,the improved paraffin section method could be used to observe iridophores in the fish skin.