DMBT1基因在上消化道癌组织中的表达及其意义

mRNA expression of DMBT1 in carcinoma of upper digestive tract and its significance

目的 探讨DMBT1基因转录表达异常在人上消化道癌组织发生、发展中的作用。方法 应用逆转录 聚合酶链反应 (RT PCR)检测 38例食管癌、2 1例贲门癌、2 5例胃癌肿瘤组织及其相应癌旁正常组织中DMBT1mRNA的表达缺失情况。结果 食管癌、贲门癌及胃癌组织中DMBT1mRNA阳性表达缺失率分别为 63 .2 % (2 4 / 38)、52 .4 % (1 1 / 2 1 )及 72 .0 % (1 8/ 2 5) ;伴有淋巴结转移的癌组织中DMBT1表达缺失率均显著高于相应淋巴结无转移的癌组织 (P <0 .0 2 5) ;肿瘤外侵越严重 ,DMBT1mRNA表达缺失率越高 (P <0 .0 2 5)。结论 DMBT1基因表达缺失与上消化道癌侵袭及淋巴结转移密切相关 ,提示该基因在上消化道癌的发生、发展及转移中起一定作用。

Objective To study the gene expression of DMBT1 in esophageal,cardiac and gastric carcinoma and its clinical pathological significance.Method The expression of DMBT1 mRNA in tumor tissues of esophageal,cardiac and gastric carcinoma and their corresponding adjacent normal tissues was detected by reverse transcriptase polymerase chain reaction (RT PCR).Results The loss rate of DMBT1 mRNA expression in esophageal,cardiac and gastric carcinomas was 63.2%(24/38),52.4%(11/21)and 72.0%(18/25),respectively. Furthermore,expression loss of DMBT1 mRNA was higher in the tumor tissues with lymph node metastasis than that without lymph node metastasis (P< 0.025). The more deeply the tumor infiltrated,the more severly the DMBT1 mRNA expression lost (P< 0.025). Conclusion DMBT1 expression was frequently lost in upper digestive tract carcinomas. Inactivation of DMBT1 may play a certain role in tumorigenesis and metastasis of these kinds of carcinomas.