犬源粪肠球菌的分离与鉴定

Isolation and identification of canine Enterococcus faecalis

随着都市宠物业的发展,伴侣动物的化脓性疾病日趋增多。为探究其影响病原,本研究对一例犬子宫蓄脓的浓汁进行了病原学鉴定。利用细菌学检测方法对采集到的病料进行分离纯化、涂片染色、镜检和生化鉴定,然后采用16S r RNA基因序列分析鉴定,发现该分离株的形态特征、生化结果与粪肠球菌一致,分离菌株的16S r RNA也与参考的粪肠球菌菌株同源性高达99%。药敏试验检测发现,该菌株对四环素、青霉素G、米诺环素、红霉素、诺氟沙星、环丙沙星、万古霉素、左氟沙星、克林霉素、多粘菌素B、氯霉素、复方新诺明和克拉霉素表现为耐药,对苯唑西林和呋喃妥因敏感。这些研究结果可为治疗此类多重耐药性肠球菌感染提供参考。

With the development of the urban pet industry,suppurative diseases of companion animals are increasing day by day.To explore its associated pathogens,this study was performed on etilolgical identification from a case of a canine uterus with pus.Bacteriological detection method was used to isolate and purify the collected disease materials,such as smear staining,microscopic examination and biochemical identification.Then,16S rRNA molecular sequence analysis was used to identify the the isolated strain.It was found that the morphological features of the isolated strain and the biochemical results were consistent with the Enterococcus faecalis.The 16S rRNA of the isolated strain was also as high as 99%homologous with the reference stains.Drug susceptibility test showed that the isolate was resistant to tetracycline,penicillin G,minocycline,erythromycin,ciprofloxacin,norfloxacin,vancomycin,levofloxacin,clindamycin,polymyxin B,chloramphenicol,compound sulfamethoxazole,and clarithromycin,and only sensitive to oxacillin and nitrofurantoin.These results provided a reference for the treatment of this type of multidrug-resistant enterococcal infection.