HPV E6/E7 mRNA与YAP在宫颈癌中的表达与相关性研究

HPV E6/E7 mRNA and YAP expression and their correlation in cervical carcinoma

目的:研究宫颈病变中HPV E6/E7 mRNA与Yes相关蛋白(YAP)的表达情况及临床意义,探讨两者之间的相关性。方法:应用支链DNA(b-DNA)技术检测36例正常宫颈组织(正常组)、45例宫颈上皮内瘤变(CIN)Ⅱ-Ⅲ级(CIN组)、43例宫颈癌(宫颈癌组)的宫颈脱落细胞标本中HPV E6/E7 mRNA的表达情况,免疫组化方法(IHC)检测上述各组患者宫颈组织石蜡包埋标本中YAP的表达情况。结果:HPV E6/E7 mRNA及YAP阳性表达率在正常组分别为16.67%、11.11%,在CIN组分别为82.22%、84.44%,在宫颈癌组分别为88.37%、90.70%,3组HPV E6/E7 mRNA及YAP阳性表达率均逐渐升高,且差异均具有统计学意义(P<0.05)。YAP在宫颈癌有淋巴结转移组中的表达明显高于无淋巴结转移组(P<0.05),而与宫颈癌的临床分期、组织学分级及组织学类型无关(P>0.05)。HPV E6/E7 mRNA与YAP的表达呈正相关(r=0.383,P<0.05)。结论:HPV E6/E7 mRNA与YAP的检出率随病理级别的升高呈增强趋势,且两者具有相关性。因此,HPV E6/E7 mRNA与YAP的联合检查,可以提高宫颈病变的诊断率并能够预测癌前病变的进展。

Objective:To investigate the expression and clinical significance of human papillomavirus(HPV)E6/E7 mRNA and Yes-associated protein(YAP)in the cervical lesions,and to explore the correlation between them.Methods:Branched DNA(b-DNA)technology was used to detect the expression of HPV E6/E7 mRNA in 36 cases of normal cervical tissues(normal group),45 cases of cervical intraepithelial neoplasia(CIN)II-III(CIN group),and 43 cases of cervical carcinoma(cervical carcinoma group).Immunohistochemistry(IHC)method was used to examine the expression of YAP in the paraffin embedded specimens of cervical tissue of the above groups.Results:The positive rates of HPV E6/E7 mRNA and YAP in normal group were 16.67%and 11.11%,82.22%and 84.44%in CIN group,and 88.37%and 90.70%in cervical carcinoma group.The positive rates of HPV E6/E7 mRNA and YAP were all gradually increased in the three groups,and the difference among the three groups was statistically significant(P<0.05).In cervical carcinoma,the expression of YAP in node-positive cases is significantly higher than node-negative cases(P<0.05),whereas no relations to clinical stage,histological grade and histological types of cervical carcinoma were found(P>0.05).The expression of HPV E6/E7 mRNA and YAP was positively correlated(r=0.383,P<0.05).Conclusion:The positive rate of HPV E6/E7 mRNA and YAP increased with the increase of pathological grade,and the expression of HPV E6/E7 mRNA and YAP was positively correlated.Therefore,the combination of HPV E6/E7 mRNA and YAP detection can improve the diagnostic rate for cervical lesions and predict the progression of precancerous lesions.