肾癌与高危型人乳头状瘤病毒基因的相关性研究

Association between high risk human papillomavirus and kidney carcinoma

目的 探讨肾癌与高危型人乳头状瘤病毒基因 (HPV1 6 ,1 8DNA)的相关性。方法 采用聚合酶链反应 (PCR)和DNA限制性内切酶酶切技术 (DNARET)对 90例成人肾恶性肿瘤组织 (其中肾透明细胞癌、肾乳头状癌、肾颗粒细胞癌各 30例 )的HPV1 6、1 8E7基因进行实验研究 ,2 0例正常肾脏组织为正常对照 ,HPV1 6、1 8阳性克隆作为阳性对照 ,去离子三蒸水做实验体系阴性对照。对每一阳性标本行限制性内切酶技术。结果 肾透明细胞癌、肾乳头状癌、肾颗粒细胞癌中HPV1 6基因表达率分别为 60 .0 %、86 .7%、63 .3 % ,总的基因表达率为 70 .0 %。HPV1 8的基因阳性率分别为 46 .7%、66 .7%、44 .3 % ,总的基因表达率为 52 .2 %。结论 高危型HPV1 6、1 8DNA与肾恶性肿瘤有一定的关系 ,尤其是HPV1 6基因表达率明显高于HPV1 8型 (P <0 .0 5) ,其中肾乳头状癌的HPV1 6、1 8基因阳性率明显高于肾透明细胞癌和肾颗粒癌 (P <0 .0 5) 。

Objective To certify the association between high risk human papillomavirus (HPV) 16/18 DNA gene and kidney carcinoma.Methods The polymerase chain reaction (PCR) using special primer and DNA restriction endonuclease technique (DNA RET) were used. Ninety renal carcinoma DNA samples were extracted from formalin fixed and paraffin embedded kidney carcinoma tissues by phenol chloroform technique,and 20 normal renal DNA samples served as negative control and HPV16,18 positive clone as positive control.Reseults Of the 90 cases of kidney carcinoma,63 (70%) were positive for HPV16 DNA expression (among them,60.0% for clear cell carcinoma of kidney,86.7% for renal papilloma,63.3% for transitional cell carcinoma of renal) and 47 (52.2%) were positive for HPV18 DNA expression (among them,46.7% for clear cell carcinoma,66.7% for renal papilloma and 44.3% for transitional cell carcinoma respectively).Conclusion There is a great correlation between high risk HPV16/18 DNA and renal carcinoma,especially HPV 16 DNA (P<0.05). HPV16/18 DNA has a higher positive gene expression in renal papilloma than in clear cell carcinoma of kidney and transitional cell carcinoma of kidney. This study also certifies that high risk HPV infection is an important carcinogenesis factor in renal carcinoma.