咖啡鞣酸抑制HSV-1感染的小胶质细胞系的炎性反应

Chlorogenic acid inhibits the inflammatory response of microglia cells infected with HSV-1

目的探究咖啡鞣酸(CGA)对单纯疱疹病毒(HSV)-1感染的小胶质细胞系BV2的抗炎作用。方法用HSV-1刺激BV2细胞建立单纯疱疹病毒性脑炎(HSE)的细胞模型,然后用不同浓度梯度的CGA进行处理;MTT法测定细胞存活率;实时定量PCR以及ELISA检测肿瘤坏死因子-α(TNF-α)和白介素(IL)-6含量及表达。结果 CGA处理后细胞存活率显著改善,在HSV-1感染BV2细胞后,CGA抑制了显著增高的TNF-α和IL-6的mRNA水平。此外,CGA减少HSV诱导的TNF-α和IL-6在细胞上清液中的释放(P<0.05)。结论 CGA抑制HSE中的炎性反应。CGA可以作为一种抗炎剂提供治疗HSE的新策略。

Objective To investigate the anti-inflammatory effect of chlorogenic acid(CGA)on BV2 microglia infected by herpes simplex virus(HSV)-1.Methods BV2 cell was stimulated by HSV-1 to create a cell model of virus encephalitis,then CGA was processed with different concentrations.MTT method was used to measure cell viability.Real-time quantitative PCR and ELISA were used to detect the content and expression of tumor necrosis factor alpha(TNF-alpha)and interleukin(IL)-6.Results After CGA treatment,the cell survival rate significantly improved,and after HSV-1 infected BV2 cells,CGA inhibited the significant increase in mRNA levels of TNF-alpha and IL-6.In addition,CGA can reduce the release of TNF-alpha and IL-6 induced by HSV in the cells.The mRNAs of TNF-alpha and IL-6 were also significantly inhibited by CGA.Conclusions CGA inhibits inflammatory response in HSE.CGA can be used as an anti-inflammatory agent to treat HSE new strategies.