新型荧光探针法检测海产品中的HSO_3^-含量

Detection of HSO_3^- in Seafood by New Fluorescence Probe Method

以菲醌和对苯二甲醛为原料合成了一种以菲并咪唑荧光探针。由于荧光探针中的醛基与HSO_3^-发生加成反应,产物的得失电子能力发生改变,分子内电荷转移(ICT),从而引起吸收光谱的变化。采用该机理测定HSO_3^-,具有反应快速、操作简便等特点,可用于海产品中HSO_3^-的测定。

Phenanthrenequinone and terephthalaldehyde work as raw materials to fabricate in which phenanthroimidazole works as matrix.Aldehyde group react with hydrosulphite(HSO-3)to produce addition product,in which electron gain or loss varies and intramolecular charge transfer(ICT)happens,leading to changes of absorption spectrum.According to this mechanism,HSO-3 could be detected.Convenience,fast response time and long lifespan are advantages of the novel fluorescent probe.