miR-106a对小鼠卵巢癌移植瘤生长的影响研究

Effect of intratumoral injection of mi R-106a inhibitor on tumor growth of ovarian cancer xenografts in mice

目的研究miR-106a对小鼠卵巢癌移植瘤生长的影响。方法选择SPF级BALB/c小鼠作为实验动物,将SKOV3卵巢癌细胞注入小鼠皮下以复制卵巢癌移植瘤模型,将模型小鼠随机分为空白对照组、阴性对照组、miR-106a抑制组,分别用不含抑制剂的转染试剂、含阴性对照抑制剂的转染试剂、含miR-106a抑制剂的转染试剂进行干预,干预前及干预后分别测定移植瘤体积以及移植瘤中PTEN信号通路分子的表达量。结果干预后10、20、30和40d时,空白对照组、阴性对照组、miR-106a抑制组之间瘤体体积的比较差异有统计学意义(P<0.05),每组内不同时间点的比较差异有统计学意义(P<0.05),组间与时间变化趋势的比较差异有统计学意义(P<0.05);干预后40d时,miR-106a抑制组小鼠肿瘤组织中PTEN的mRNA表达高于空白对照组和阴性对照组,PI3K、AKT、CyclinD1、Survivin、MMP-2、MMP-9、VEGF的mRNA表达低于空白对照组和阴性对照组。结论miR-106a对小鼠卵巢癌移植瘤的生长具有抑制作用,增强PTEN信号通路是miR-106a抑制肿瘤生长的分子机制。

Objective To study the effect of intratumoral injection of miR-106a inhibitor on tumor growth of ovarian cancer xenografts in mice.Methods SPF BALB/c mice were selected as the experimental animal,then ovarian cancer xenograft model was made by injection of ovarian cancer SKOV3 cells into nude mice.The model mice were randomly divided into a blank control group,a negative control group and a miR-106 inhibition group,intervented respectively by transfection of reagent containing no inhibitor,transfection of reagent containing negative control inhibitors and transfection of reagent containing miR-106a inhibitor.Before and after intervention,the xenograft volume and expressions of PTEN signaling molecules in the xenografts were measured.Results On the 10th,20th,30th and 40th d after intervention,there were significant differences in the tumor volume among the 3 groups(P<0.05),and there were significant differences in the tumor volume at different time points in each group(P<0.05).The change trends of the tumor volume with time had statistical differences among the 3 groups(P<0.05).On the 40th d after intervention,PTEN mRNA expression level of the miR-106a inhibition group was significantly higher than that of the blank control group and the negative control group(P<0.05),while the mRNA expression levels of PI3K,AKT,Cyclin D1,Survivin,MMP-2,MMP-9 and VEGF were significantly lower than those of the blank control group and the negative control group(P<0.05).Conclusions Intratumoral injection of miR-106a inhibitor has inhibitory effect on tumor growth of ovarian cancer xenografts in mice,and the enhancement of PTEN signaling pathway is the molecular mechanism of tumor growth inhibition by miR-106a inhibitor.