鼻咽癌筛查诊断中应用鼻咽部渗液苯胺蓝染色技术的效果观察

Application of nasopharyngeal percolation aniline blue staining in screening and diagnosis of nasopharyngeal carcinoma

目的观察鼻咽部渗液苯胺蓝染色技术对鼻咽癌筛查和诊断价值。方法随机选择2015年1月~2017年1月至我院诊断为鼻咽癌的患者100例作为实验组,所有患者均经病理检查确定诊断。随机选择同期至我院耳鼻喉科检查,患有易致鼻咽部出血但非鼻咽癌的患者100例作为对照组。所有患者均行鼻咽部渗液肿瘤细胞原血红素(TCPH)检查。以病理诊断结果为最终诊断,观察实验组TCPH检测的阳性诊断结果,灵敏度、特异度以及阳性诊断符合率。观察两组患者TCPH检测的阳性结果是否有统计学差异。结果实验组患者100例病理诊断为鼻咽癌患者,TCPH诊断阳性96例,阴性结果 4例,阳性率为96.00%,以病理诊断为标准,其阳性诊断符合率96.00%与病理诊断无明显差异(χ2=2.296,P=0.130);病理诊断为鼻咽癌与鼻炎检查无异常发现或非癌病例TCOH检测率计较,差异有统计学意义(P<0.05);实验组患者TCPH阳性率为96.00%,明显高于对照组患者16.00%,且差异有统计学意义(P<0.05)。结论鼻咽部渗液苯胺蓝染色技术对于鼻咽癌筛查和诊断具有重要的临床应用价值,其操作简便,阳性率符合率高,能够为鼻咽癌的诊断提供可靠的依据。

Objective To observe the value of nasopharyngeal percolation aniline blue staining in screening and diagnosis of nasopharyngeal carcinoma.Methods One hundred cases of nasopharyngeal carcinoma from January 2015 to January 2017 in our hospital were selected as experimental group.All patients were diagnosed by pathological examination.One hundred patients with nasopharyngeal bleeding without nasopharyngeal carcinoma were randomly selected as control group.All patients were examined by Hemorheology(TCPH)of nasopharyngeal exudate tumor cells.The results of pathological diagnosis were the final diagnosis.The positive diagnostic results of TCPH in the experimental group were observed,the sensitivity,specificity and positive diagnostic coincidence rate were observed.The statistical difference of the positive results of TCPH test between the two groups was observed.Results In the experimental group,100 patients were diagnosed as nasopharyngeal carcinoma,96 cases were diagnosed positive by TCPH,4 cases were negative,the positive rate was 96.00%,according to pathological diagnosis,the coincidence rate of positive diagnosis was 96.00%,and there was no significant difference in pathological diagnosis(χ2=2.296,P=0.130).There was a statistically significant difference in the detection rate of TCOH between nasopharyngeal carcinoma diagnosed by pathological examination and rhinitis without abnormal or non cancer cases(P<0.05).The positive rate of TCPH in the experimental group was 96.00%,which was significantly higher than that in the control group(16.00%),and the difference was statistically significant(P<0.05).Conclusion The technique of aniline blue staining of nasopharyngeal fluid has important clinical value for the screening and diagnosis of nasopharyngeal carcinoma.Its operation is simple and the positive rate is high.It can provide a reliable basis for the diagnosis of nasopharyngeal carcinoma.