大黑山薏苡草粉替代苜蓿草粉对生长肉兔血清生化指标、肠道免疫和抗氧化功能的影响

Effects of Replacement of Alfalfa Meal by Coix lacryma-jobi cv.Daheishan Meal on Serum Biochemical Parameters,Intestinal Immunity and Antioxidant Capacity of Growing Rabbits

本试验旨在研究不同比例的大黑山薏苡草粉替代饲粮中苜蓿草粉对生长肉兔血清生化指标、脏器相对重及肠道免疫和抗氧化功能的影响。100只体重相近的35日龄断奶新西兰白兔随机等分为5组(每组10个重复,每个重复2只),分别饲喂5种等能、等氮、等纤维饲粮,包括1种对照(C)和4种测试饲粮(S25、S50、S75和S100,大黑山薏苡草粉分别替代对照饲粮中25%、50%、75%和100%苜蓿草粉)。试验期29 d。于第15天和第29天从每组选4只肉兔采血和屠宰采集组织器官样品。结果显示:1)除第15天的血清尿素氮含量组间有显著差异(P<0.05)外,其余所测血清生化指标各组间差异不显著(P>0.05);2)所测脏器相对重各组间均无显著差异(P>0.05);3)十二指肠、空肠和回肠黏膜中的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性及分泌型免疫球蛋白A(SIg A)和黏蛋白2(MUC2)含量各组间均无显著差异(P>0.05),但总体上测试组SOD和CAT活性在数值上高于C组,尤其是S50组。由此可见,大黑山薏苡草粉替代饲粮中苜蓿草粉对生长肉兔的生理机能无负面影响,且略有提高肠道抗氧化功能的作用,尤其是50%的替代比例。

The objective of this study was to investigate the effects of substituting different proportion of Coix lacryma-jobi cv.Daheishan meal for alfalfa meal in the diet for growing rabbits on serum biochemical parameters,organ relative weight,intestinal immune and antioxidant capacity.A total of 100 weaned New Zealand white rabbits(35 days of age)with similar body weight were randomly assigned to 5 groups with 10 replicates and 2 rabbits per replicate.Rabbits in 5 groups were fed isoenergetic,isonitrogenous and isofibrous diets,including 1 control diet(C)and 4 test diets with 25%(S25),50%(S50),75%(S75)and 100%Coix lacryma-jobi cv.Daheishan meal to replace alfalfa meal in control diet(S100),respectively.The experiment lasted for 29 d.On the 15th and 29th day of the experiment,blood,tissue and organ and tissue samples were collected from 4 rabbits for each group.The results showed as follows:1)there were no significant differences in serum biochemical parameters among groups(P>0.05),except for the urea nitrogen content on the 15th day(P<0.05).2)Organ relative weight of rabbits in five different groups showed no significant differences(P>0.05).3)No significant differences were found on the activities of superoxide dismutase(SOD)and hydrogen peroxidase(CAT)and the contents of secreted immunoglobulin A(SIgA)and mucin 2(MUC2)in duodenal,jejunal and ileal mucosa among groups(P>0.05),although the activities of SOD and CAT for four test groups(particularly for S50 group)showed generally higher than group C.Collectively,Coix lacryma-jobi cv.Daheishan meal can replace dietary alfalfa meal without negatively affecting physiological function and slightly improving intestinal antioxidant capacity of growing rabbits,especially better when the replacement proportion is 50%.