酵母浸粉对家蚕血淋巴中甘油三酯含量和尿酸代谢的影响

Effects of Yeast Extract Powder on Triglyceride Content and Uric Acid Metabolism in Hemolymph of Silkworms

本试验旨在研究酵母浸粉对家蚕血淋巴中甘油三酯含量和尿酸代谢的影响。以蚕品种"菁松×皓月"为试验材料,挑选大小一致、发育整齐的家蚕600头,随机分成4个组(每组3个重复,每个重复50头,雌雄各占1/2),从5龄起蚕开始,3个试验组(JM1组、JM2组、JM3组)分别饲喂以1%、2%和4%酵母浸粉溶液涂抹的新鲜桑叶,对照组则以蒸馏水替代酵母浸粉溶液,分别在添食酵母浸粉3、4、5 d后检测家蚕血淋巴中尿酸含量、甘油三酯含量以及黄嘌呤氧化酶(XOD)活性。结果表明:添食不同浓度酵母浸粉5 d后家蚕体重增长率均较对照组显著升高(P<0.05),但各试验组之间差异不显著(P>0.05),且随着酵母浸粉浓度的升高,体重增长率呈现下降的趋势。添食不同浓度酵母浸粉5 d后家蚕血淋巴中甘油三酯含量比对照组增加42%~98%,其中JM 1组、JM 2组与对照组的差异达到显著水平(P<0.05);随着添食时间的延长,各组家蚕血淋巴中尿酸含量均呈现下降趋势,第5天与第3天相比差异均达到显著水平(P<0.05);随着酵母浸粉浓度的升高,各时间点家蚕血淋巴中尿酸含量均呈现升高趋势,但各试验组与对照组的差异均未达到显著水平(P>0.05)。3个试验组家蚕血淋巴中XOD活性在各时间点均显著高于对照组(P<0.05),但第3、4天家蚕血淋巴中XOD活性随着酵母浸粉浓度的升高均呈现先上升后下降趋势。家蚕血淋巴中尿酸含量与XOD活性之间存在显著或极显著的正相关性(P<0.05或P<0.01),相关系数在0.652~0.902。由此得出,添食一定浓度的酵母浸粉可导致家蚕血淋巴中尿酸含量、甘油三酯含量以及黄嘌呤氧化酶(xanthine oxidase,XOD)活性升高,从而影响家蚕的尿酸代谢。

This experiment was conducted to investigate the effects of yeast extract powder(YEP)on the con-tent of triglyceride and the metabolism of uric acid(UA)in hemolymph of silkworms(Bombyx mori).The silkworm variety“Jingsong×Haoyue”was used as experimental material.Choosing similar size and develop-ment of 600 silkworms randomly divided into four groups with each group had three replicates and each repli-cate had 50 silkworms(half male and half female).Fifth instar larvae of silkworms in experimental groups(groups JM1,JM2 and JM3)were fed fresh mulberry leaves painted with 1%,2%and 4%YEP solution,re-spectively,and those in control group were fed fresh mulberry leaves painted with distilled water.The UA con-tent,triglyceride(TG)content and xanthine oxidase(XOD)activity in hemolymph of silkworms were detec-ted after feeding with different concentrations of YEP for 3,4 and 5 days.The results showed that the body weight growth rate of silkworms increased significantly after feeding with different concentrations of YEP for 5 days compared with the control group(P<0.05),but no significant differences were found among experimen-tal groups(P>0.05),and with the increase of YEP concentration,the body weight growth rate showed a trend of decline.The content of TG in hemolymph of silkworms after feeding with different concentrations of YEP for 5 days was 1.42 to 1.98 times higher than that of control group,and the differences between the control group and the groups JM1 or JM2 were significant(P<0.05).The content of UA in hemolymph of silkworms of each group showed a trend of decline with the extension of time.Compared with the 3rd day,the content of UA in hemolymph of silkworms of each group was significantly decreased compared with the 5th day(P<0.05).The content of UA in hemolymph of silkworms of each time point showed a trend of increase with the increase of YEP concentration,but the differences were not significant between the control group and the ex-perimental groups(P>0.05).The activity of XOD in hemolymph of silkworms of each time point was signifi-cantly higher than that of control group(P<0.05),but it was firstly increased and then decreased with the in-crease of YEP concentration.There was a significant or an extremely significant correlation between the content of UA and the activity of XOD in hemolymph of silkworms(P<0.05 or P<0.01),and the correlation coeffi-cient ranged from 0.652 to 0.902.It is concluded that feeding a certain concentration of YEP can lead to increa-ses of UA content,TG content and XOD activity in hemolymph,thus affecting the UA metabolism of silk-worms.