高粱型饲粮中添加复合酶和益生菌对良凤花肉鸡生长性能、血清抗氧化指标及肠道结构的影响

Effects of Supplementation with Compound Enzymes and Probiotics on Sorghum Diets on Growth Performance,Serum Antioxidant Indexes and Intestinal Morphology of Liangfenghua Broilers

本试验旨在研究高粱型饲粮中添加复合酶和益生菌对良凤花肉鸡生长性能、血清抗氧化指标及肠道结构的影响。试验选取1日龄体重相近的健康良凤花肉鸡公鸡900只,随机分为5个组,每组6个重复,每个重复30只鸡。对照组饲喂基础饲粮,试验组分别饲喂高粱型饲粮(高粱组)、高粱型饲粮+复合酶(高粱+复合酶组)、高粱型饲粮+益生菌(高粱+益生菌组)和高粱型饲粮+复合酶+益生菌(高粱型+复合酶+益生菌组)。试验期56 d,分为试验前期(1~28日龄)和试验后期(29~56日龄)。试验结束后测定试验鸡生长性能、血清抗氧化指标和肠道形态。结果表明:1)试验前期,各试验组肉鸡平均日采食量(ADFI)和料重比(F/G)均显著低于对照组(P<0.05);试验后期和试验全期(1~56日龄),除高粱+复合酶组外,其余各试验组肉鸡ADFI和平均日增重(ADG)均显著低于对照组(P<0.05)。2)与对照组相比,第28天高粱组肉鸡血清总超氧化物歧化酶(T-SOD)活性显著降低(P<0.05),其余各组之间无显著差异(P>0.05);第56天各组之间肉鸡血清抗氧化指标均差异不显著(P>0.05)。3)第28天,除高粱组外,其他各试验组肉鸡十二指肠绒毛高度和绒毛高度/隐窝深度(V/C)值均显著高于对照组(P<0.05);第56天,各试验组肉鸡十二指肠、空肠、回肠的绒毛高度、隐窝深度及V/C值与对照组均无显著差异(P>0.05)。综上所述,在饲粮中用高粱替代玉米,显著降低了良凤花肉鸡的生长性能,而在高粱型饲粮中添加复合酶能获得与对照组相当的生长性能,在高粱型饲粮中添加复合酶和益生菌对良凤花肉鸡的肠道形态结构和血清抗氧化指标均无不良影响。

This experiment was conducted to investigate the effects of supplementation with compound enzymes and probiotics on sorghum diets on growth performance,serum antioxidant indexes and intestinal morphology of Liangfenghua broilers.A total of 900 healthy one-day-old Liangfenghua male broilers with similar body weight were randomly selected and allocated to 5 groups with 6 replicates per group and 30 broilers per repli-cate.Broilers in the control group were fed a basal diet,and others in the experimental groups were fed sor-ghum diet(sorghum group),sorghum diet+compound enzyme(sorghum+compound enzyme group),sor-ghum diet+probiotics(sorghum+probiotics group)and sorghum diet+compound enzyme diet+probiotics(sor-ghum+compound enzyme+probiotics group),respectively.The experiment lasted for 56 days,and divided in-to two stages of early stage(1 to 28 days of age)and later stage(29 to 56 days of age).At the end of experi-ment,the growth performance,serum antioxidant indexes and intestinal morphology of broilers were evaluated.The results showed as follows:1)at the early stage,the average daily feed intake(ADFI)and feed to gain ratio(F/G)of broilers in experimental groups were significantly lower than those in control group(P<0.05).At the later stage and the whole stage(1 to 56 days of age),the ADFI and the average daily gain(ADG)of broilers in experimental groups(except sorghum+compound enzyme group)were significantly lower than those in control group(P<0.05).2)Compared with the control group,the serum total superoxide dismutase(T-SOD)activity of broilers at 28 days of age in sorghum group was significantly decreased(P<0.05),and no significant difference was observed among the rest groups(P>0.05).At 56 days of age,there were no sig-nificant differences on serum antioxidant indexes of broilers among all groups(P>0.05).3)At 28 days of age,the villus height and villus height/crypt depth(V/C)value in duodenum of broilers experimental groups(except畩*p-gsorghum group)were significantly higher than those in control group(P<0.05).At 56 days of age,there were no significant differences on villus height,villus height and V/C value in duodenum,jejunum and ileum of broilers among all groups(P>0.05).In conclusion,sorghum instead of corn in the diet significantly reduce the growth performance of Liangfenghua broilers,and added compound enzyme in the sorghum diet can obtaine the similar growth performance compared the control group,the sorghum diet supplemented with com-pound enzyme and probiotics have no adverse effects on intestinal morphologic structure and serum antioxidant indexes.