摘要
Simple sequence repeat(SSR) capillary electrophoresis and single nucleotide polymorphism(SNP) array are widely used tools for investigating genetic diversity. However, efficiency between SSR and SNP on rapeseed genetic diversity has not been systematically assessed yet. In this study, both SSR and SNP were used on 446 worldwide B. napus germplasm lines. Data shows that 65 pairs of primers(70 SSRs) and 250 SNPs were necessary to identify the similar accessions. Furthermore, no significant differences were found between 2 systems on basic statistics, population structures, principal components and kinship parameters. In general, either SSR or SNP is efficient for genetic diversity estimation. However, our data revealed the fact that SNP array system shows slightly more accuracy than SSR system on ecotype groups division.
Simple sequence repeat(SSR) capillary electrophoresis and single nucleotide polymorphism(SNP) array are widely used tools for investigating genetic diversity. However, efficiency between SSR and SNP on rapeseed genetic diversity has not been systematically assessed yet. In this study, both SSR and SNP were used on 446 worldwide B. napus germplasm lines. Data shows that 65 pairs of primers(70 SSRs) and 250 SNPs were necessary to identify the similar accessions. Furthermore, no significant differences were found between 2 systems on basic statistics, population structures, principal components and kinship parameters. In general, either SSR or SNP is efficient for genetic diversity estimation. However, our data revealed the fact that SNP array system shows slightly more accuracy than SSR system on ecotype groups division.
基金
supported by National Key R & D Program"Accurate identification and innovative utilization of main cash crop germplasm resources" (2016YFD0100202)
