珍珠岩引发对盐胁迫下青椒种子萌发和幼苗生长的影响

Effects of Perlite Priming on the Seed Germination and Seedling Growth of Pepper under Salt Stress

【目的】探究珍珠岩引发对盐胁迫下青椒种子萌发特性和幼苗耐盐性的影响。【方法】通过珍珠岩引发处理两种青椒(精细翠长、辣艳12号)种子,并设定3个Na Cl浓度(0、100、200 mmol/L)模拟盐胁迫,测定不同处理下辣椒种子的萌发指数和幼苗干鲜重、3种抗氧化酶SOD、POD和CAT的活性,丙二醛(MDA)含量及3种渗透调节物质(游离脯氨酸、可溶性蛋白质、可溶性糖)的含量。【结果】引发处理下辣椒种子的萌发指数和幼苗干重、鲜重均高于相应的对照,经过引发处理后的两种青椒幼苗的SOD、POD和CAT的活性显著高于对照(P<0.05);而青椒幼苗的膜脂过氧化产物MDA含量则呈现出逐渐降低的趋势;游离脯氨酸、可溶性蛋白质和可溶性糖的含量均高于对照。【结论】珍珠岩引发能够提高两种辣椒种子的活力并增强青椒幼苗的耐盐性。

[Purpose]Our aim was to study the effects of perlite priming on the germination characteristics and salt tolerance of pepper seeds under salt stress.[Method]We used the perlite to deal with two kinds of peppers(JXCC,Layan 12)seeds and set three NaCl concentration(0,100,200 mmol/L)simulation salinity stress to study the pepper seed germination under salt stress characteristic and the influence of salt resistance.[Results]Under the perlite processing the seed germination index,seedling dry weight and fresh weight were higher than those of the corresponding control.Meanwhile,perlite trigger treatment could significantly promote the activities of three protective enzymes(SOD,POD and CAT)(P<0.05).The membrane lipid peroxide malondialdehyde(MDA)content of green pepper seedlings gradually reduced.The free proline,soluble protein and soluble sugar contents were higher than those of control.[Conclusion]The above results showed that pearlite trigger could enhance the vitality of two kinds of green pepper seeds and enhance green pepper seedlings salt resistance.