高产四甲基吡嗪芽孢杆菌的筛选及在清香型原酒生产中的应用研究

Screening of Bacillus Strains with High-Yield of Tetramethylpyrazine and Its Application in Qingxiang Base Liquor Production

四甲基吡嗪作为白酒中的重要呈味物质,近年来得到白酒企业的广泛关注。从大曲中分离到11株芽孢杆菌,通过脱脂奶粉平板初筛,发酵复筛,得到了4株高产四甲基吡嗪的芽孢杆菌。优选的菌株应用于原酒酿造,极大提高了原酒中四甲基吡嗪的含量,大曲酒中四甲基吡嗪含量达到10 mg/L以上。

Tetramethylpyrazine(TTMP),as the important taste-producing substance,has received extensive attention in distilleries in recent years.In this study,11 Bacillus strains were isolated from Daqu.After preliminary screening with skimmed milk powder as the culture medium and secondary screening by fermentation,4 Bacillus strains with high-yield of TTMP were finally obtained.These strains were applied in Qingxiang base liquor production.As a result,TTMP content in base liquor got improved greatly,reaching up to over 10 mg/L.