非小细胞肺癌中ZEB1和FoxO3a表达及意义

Expression and Significance of ZEB1 and FoxO3a in Non-small Cell Lung Cancer

目的研究E盒结合锌指蛋白1和叉头框家族蛋白在非小细胞肺癌组织中的表达,并探讨其表达对非小细胞肺癌浸润、转移的影响。方法收集2013年6月~2015年6月沧州市中心医院手术切除的130组非小细胞肺癌肿瘤组织和癌旁正常组织,免疫组织化学法和免疫印迹法检测E盒结合锌指蛋白1和叉头框家族蛋白蛋白的表达,分析E盒结合锌指蛋白1和叉头框家族蛋白与非小细胞肺癌临床资料的关系。结果肿瘤组织E盒结合锌指蛋白1蛋白表达显著高于癌旁正常组织(P<0.05),而叉头框家族蛋白蛋白表达显著低于癌旁正常组织(P<0.05);E盒结合锌指蛋白1和叉头框家族蛋白蛋白表达均与肿瘤大小、分化程度、淋巴结转移、远端转移以及TNM分期显著相关(P<0.05);在淋巴结转移或远端转移的非小细胞肺癌患者中,E盒结合锌指蛋白1蛋白表达显著升高(P<0.05),叉头框家族蛋白蛋白表达显著降低(P<0.05);高表达E盒结合锌指蛋白1或者低表达叉头框家族蛋白的非小细胞肺癌患者术后2年生存率显著降低。结论 E盒结合锌指蛋白1在非小细胞肺癌组织中高表达,叉头框家族蛋白在非小细胞肺癌组织中低表达,两者协同促进非小细胞肺癌浸润转移。

Objective To study the expression of zinc finger E binding protein 1 and forkhead box family proteins in non small cell lung cancer,and to investigate the expression of non small cell lung cancer,metastasis.Methods From June 2013 to June 2015,130 groups of non-small cell lung cancer(NSCLC)tumor tissues and adjacent normal tissues were collected from Cangzhou central hospital.Immunohistochemistry and western blotting were used to detect the expression of E-box binding zinc finger protein 1 and fork frame family protein.To analyze the relationship between E-box binding zinc finger protein 1 and fork box family protein and clinical data of non-small cell lung cancer.Results The expression of E-box binding zinc finger protein-1 protein in tumor tissues was significantly higher than that in adjacent normal tissues(P<0.05),while the protein expression of fork box family protein was significantly lower than that in adjacent normal tissues(P<0.05).The expression of E-box binding zinc finger protein 1 and fork box family protein were significantly correlated with tumor size,differentiation,lymph node metastasis,distal metastasis and TNM stage(P<0.05).In non-small cell lung cancer patients with lymph node metastasis or distant metastasis,the expression of E-box combined with zinc finger protein 1(P<0.05)and protein of fork-framed family protein decreased significantly(P<0.05).High expression patients with non-small-cell lung cancer with E-box combined with zinc finger protein 1 or with low expression of fork-frame family protein had a significantly lower 2-year postoperative survival rate.Conclusion The E-box combined with zinc finger protein 1 is highly expressed in non-small cell lung cancer tissues,and the forkhead box family protein is low expressed in non-small cell lung cancer tissues.Both of them can promote the invasion and metastasis of non-small cell lung cancer.