姜黄素对罗曼蛋鸡血液和组织抗氧化性能的影响

Effects of Curcumin on the Antioxidant Properties in the Blood and Tissues of Roman Laying Hens

[目的]探讨日粮中添加不同剂量姜黄素对罗曼蛋鸡血液和组织抗氧化性能的影响。[方法]选取25周龄健康、生产性能相近的罗曼粉壳蛋鸡288只,分为对照组(CK)和5个处理组(C1、C2、C3、C4、C5),每组4个重复,每个重复12只鸡,对照组饲喂基础日粮,处理组饲喂基础日粮+不同剂量(100、150、200、250、300 mg/kg)姜黄素,分析姜黄素对蛋鸡血液、组织抗氧化性能的影响。[结果]在血液抗氧化方面,试验期间在各处理组添加不同剂量的姜黄素均能显著提高过氧化氢酶(CAT)活性(P<0.05);C3、C4和C5组的谷胱甘肽过氧化物酶(GSH-Px)活性均显著高于对照组(P<0.05),C5组的GSH-Px活性最高;在试验后期各处理组的GSH-Px活性均显著提高(P<0.05);各处理组的丙二醛(MDA)含量均显著低于对照组(P<0.05);各处理组的超氧化物酶(SOD)活性均显著高于对照组(P<0.05),C4组SOD活性最高。在组织抗氧化方面,试验期间C3组肝脏中SOD活性和T-AOC显著提高(P<0.05);C2组肝脏中MDA含量最低。各处理组心脏的SOD活性均显著提高(P<0.05),C4组SOD活性最高;C3组心脏总抗氧化能力(T-AOC)最高;C4组心脏中MDA含量最低;C3、C4组肺脏中SOD活性显著提高(P<0.05);与对照组相比,各处理组肺脏GSH-Px的活性均显著提高(P<0.05),C3组GSH-Px活性最高。[结论]姜黄素可以提高抗氧化酶CAT、SOD以及GSH-Px的活性,不同剂量姜黄素均能起到改善蛋鸡机体抗氧化性能的作用。饲粮中添加200 mg/kg姜黄素时抗氧化效果较好。

[Objective]To discuss the effects of adding different doses of curcumin in the diet on the antioxidant properties in the blood and tissues of Roman laying hens.[Method]25-week-old 288 healthy pink-shell Roman laying hens with similar production performance were selected and divided into control group(CK)and five treatment groups(C1,C2,C3,C4 and C5).There were 4 repetitions in each group,and there were 12 chicken in each repetition.The chicken were fed with the basal diet,and those in five treatment groups(C1,C2,C3,C4 and C5)were fed with basal diet+different doses(100,150,200,250,200 mg/kg)of curcumin.The effects of curcumin on the antioxidant properties in the blood and tissues of Roman laying hens were analyzed.[Result]As for antioxidant properties in the blood,adding different doses of curcumin in each treatment group could enhance CAT activity during the experiment period(P<0.05).The activity of GSH-Px in C3,C4 and C5 groups were significantly higher than that in CK group(P<0.05),GSH-Px activity in C5 group was the highest.In late experiment period,GSH-Px activity in each treatment group was significantly improved(P<0.05).MDA content in each treatment group was significantly lower than that in CK group(P<0.05).SOD activity in each treatment group was significantly higher than that in CK group(P<0.05)and that in C4 group was the highest.As for antioxidant properties in the tissues,SOD activity and total antioxidant capacity(T-AOC)in the liver in C3 group were significantly improved during the experiment period.MDA content in the liver in C2 group was the lowest.SOD activity in the heart in each treatment group was significantly improved(P<0.05)and SOD activity in C4 group was the highest.T-AOC in the heart in C3 group was the highest.MDA content in the heart in C4 group was the lowest.SOD activity in the lung in C3 and C4 groups were significantly increased(P<0.05).Compared with CK group,GSH-Px activity in the lung in each treatment group were significantly increased(P<0.05)and that in C3 group was the highest.[Conclusion]Curcumin could improve the activities of antioxidant enzymes(CAT,SOD and GSH-Px).Different doses of curcumin could improve the antioxidant properties of laying hens.The antioxidant effect of adding 200 mg/kg curcumin in the basal diet was better.