摘要
为了促进环介导等温扩增(LAMP)技术的推广应用,在传统LAMP反应体系中引入羟基萘酚蓝指示剂,建立可视LAMP方法,对反应体系进行高温-低温前处理和添加甜菜碱等优化措施提高检测敏感性,液面覆盖矿物油降低气溶胶污染,据此建立的猴沙门菌可视LAMP检测方法敏感度为6CFU/反应(25μL体系),结果判定直观明了,与其他细菌无非特异性反应,检验效率和检出率均优于细菌培养鉴定法。
In order to promote the application of LAMP technology,the indicator hydroxynaphthol blue was added to reaction system of the traditional LAMP in the study.The reaction system was prepared by heating-cooling pretreatment,and betaine was added to the reaction system to increase the sensibility.The liquid surface was covered by mineral oil to decrease aerosol contamination.Base on those above,we built a visible loop-mediated amplification method for detecting experimental monkey Salmonella.The sensibility of the visible LAMP is 6 CFU/reaction.The visible LAMP showed intuitive and specific results,more efficient and higher detection rate than germiculture.
作者
温和心
龙英全
蒋荣华
盘宝进
罗广生
陈立军
WEN He-xin;LONG Ying-quan;JIANG Rong-hua;PAN Bao-jin;LUO Guang-shen;CHEN Li-jun(Guigang Entry-exit Inspection&Quarantine Bureau,Guigang,Guangxi,537100,China;National Key Primate Laboratory,Nanning,Guangxi,530000,China;Yulin Entry-exit Inspection&Quarantine Bureau,Yulin,Guangxi,530002,China)
出处
《动物医学进展》
北大核心
2018年第8期40-44,共5页
Progress In Veterinary Medicine
基金
国家质检总局科技计划项目(2016IK072)
桂科能1598025-26
关键词
可视环介导等温扩增
检测
沙门菌
visible loop-mediated amplification
detection
Salmonella
