外源性供氧对脊髓损伤后继发性脊髓水肿的影响

The effect of exogenous oxygen treatment after spinal cord trauma on secondary spinal cord edema in rabbits

目的 观察外源性供氧对脊髓损伤组织学的影响。方法 采用Allen法于兔L2 椎体水平造成急性脊髓损伤。对照组不予治疗 ,携氧复合液治疗组和复合液治疗组兔分别在伤后 30min开始给药。于伤后 48h、3、7d、4周分别从各组中取 5只兔 ,进行脊髓组织含水量、丙二醛(MDA)及形态学观察。结果 伤后 48h、3、7d携氧复合液组伤段脊髓组织含水量分别为 (69.1 4±0 .92 ) %、(71 .93± 0 .87) %、(71 .63± 1 .0 1 ) % ;脊髓组织MDA含量分别为 (2 8.54± 5 .98) μmol/g、(2 7.81± 3 .64) μmol/g、(2 6.1 4± 4 .2 6) μmol/g,均显著低于对照组 (P <0 .0 1 )。组织形态学检查携氧复合液组的病理改变较对照组轻。结论 脊髓损伤后早期外源性供氧对继发性脊髓损伤有保护作用。

Objective To observe the effects of exogenous oxygen treatment on the change of histology after spinal cord injury in rabbits.Methods An injured model of L 2 spinal cord was produced by the Allen's method.The control group received no treatment.Oxygenated compound liquid was give in one group,and compound liquid was given in another group intravenously 30 min after injury.Five rabbits in the test and control group each were sacrificed successively at 48?h,3?d,7?d,4 weeks,after trauma.The amount of MDA and the water content of spinal tissue were determined,and pathomorphological changes of spinal cord were observed.Results The water content of spinal cord within 48?h,3?d,7?d was (69.14±0.92)%,(71.93±0.87)%,and(71.63±1.01)% respectively in the oxygenated compound liquid group;MDA was (28.54±5.98)?μmol/g,(27.81±3.64)?μmol/g,and (26.14±4.26)?μmol/g respectively,lower significantly than in the control one (P< 0.01). Morphologic findings of oxygenated compound liquid group revealed slighter degeneration and necrosis in gray and white matter than in control group.Conclusion Exogenous oxygen treatment had protective effects on the secondary spinal cord edema induced by the trauma.