GC-MS法检测牛可食性组织中克仑特罗残留量的测定不确定度评定

Uncertainty Evaluation of Determination of Clenbuterol Residues in Bovine Edible Tissues by Gas Chromatography Mass Spectrometry

建立了气相色谱/质谱法测定牛肉中克仑特罗含量的不确定度分析方法,以评价测定结果的准确性。以测量不确定度的评定理论为依据,建立了数学模型,分析标准《农业部958号公告-8-2007》测定过程中的不确定度来源,系统计算不确定度分量。评定结果表明,该方法不确定度的主要来源为拟合直线求样品溶液浓度和标准溶液配制;当样品中克仑特罗含量为10.70μg/kg时,其扩展不确定度为(10.70±0.81)μg/kg(k=2)。本文建立不确定度评价方法适用于气相色谱/质谱法测定牛肉中克仑特罗含量的不确定度分析。

An uncertainty analysis method for determination of clenbuterol in beef was established by gas chromatography/mass spectrometry(GC/MS)to evaluate the accuracy of the results.Based on the evaluation theory of uncertainty measurement,a mathematical model was established,the source of uncertainty in the standard“958 bulletin-8-2007 of the Ministry of agriculture”was analyzed,and the uncertainty component of the system was calculated.The evaluation results showed that the main source of the uncertainty of the method was the sample solution concentration and the standard solution.When the content of clenbuterol in the sample was 10.70 g/kg,the expansion uncertainty was(10.70+0.81)g/kg(k=2).The uncertainty evaluation method was suitable for the determination of clenbuterol in beef by gas chromatography/mass spectrometry(GC/MS).