枣果皮细胞壁代谢酶活性与抗裂果的关系

Study on the Relationship between the Activity of Cell Wall Metabolic Enzymes and Anti-Cracked in Jujube Peel

【目的】测定枣果皮细胞壁代谢酶活性,研究果皮细胞壁代谢酶活性与抗裂果的关系。【方法】以抗裂枣品种扁核酸与易裂枣品种京枣39为研究对象,测定生长过程中枣果皮不同部位细胞壁代谢酶活性,比较抗裂和易裂枣品种果皮细胞壁代谢酶活性的差异。【结果】抗裂枣品种不同时期不同部位果皮的超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、多酚氧化酶(PPO)活性显著或极显著高于易裂枣品种,而在绿熟期不同部位抗裂品种的过氧化氢酶(CAT)活性极显著低于易裂品种。【结论】易裂品种果皮果肩阴面POD活性和PPO活性在白熟期显著高于抗裂品种,而其余时期其余部位则是抗裂品种的POD活性和PPO活性显著高于易裂品种,易裂品种果皮的梗洼、果顶、果肩阳面和果肩阴面CAT活性在绿熟期极显著高于抗裂品种,而其余时期则是抗裂品种高于易裂品种。

【Objective】With the flat nucleic acid in anti-split date varieties and Jing jujube 39 in easily cracked date varieties as study subjects,cell wall metabolic enzyme activity in the pericarp of jujube was determined to reveal the relationship between cell wall metabolic enzyme activity and split-resistant fruit.【Method】The activity of cell wall metabolic enzymes in different parts of jujube peel was determined during the growth process,and the difference of cultivars cell wall metabolic enzyme activity between split-resistant and split jujube was compared.【Result】Results showed that the activities of superoxide dismutase,peroxidase and polyphenol oxidase in the split-resistant cultivars pericarp were significantly or extremely significantly higher than those of susceptible jujube varieties during the different periods,while catalase activity was significantly lower in different parts at the green maturity stage.【Conclusion】POD and PPO activities in the shoulder nightside of easily-cracked cultivars pericarp were extremely significantly lower than those of crack-resistant cultivars at white mature stage,while at the rest time,POD,PPO activities in other parts of crack-resistant cultivars were extremely significantly higher in the different parts at different time than those easily-cracked cultivars pericarp.CAT activity was significantly lower in different parts at Green time,but it was higher at different time.The activity of CAT in shoulder nightside of pericarp of susceptible cultivars was significantly higher than that of resistant cultivars at green maturity stage,while that of resistant cultivars was higher than that of susceptible cultivars in other periods.