基于网络药理学方法对脑泰方干预脑梗死的作用机制研究

Mechanism of Action of Naotaifang in Treatment of Cerebral Infarction:A Study Based on Network Pharmacology

目的筛选脑泰方主要有效成分,预测其活性成分干预脑梗死的靶点及通路,对比脑梗死有效靶点及涉及的通路,探究脑泰方干预脑梗死的作用机制。方法采用检索中药系统药理学技术平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)、台湾中医药资料数据库以及文献资料筛选出脑泰方的主要有效成分,利用其活性成分基于Pubchem数据库和Swiss Target Prediction数据库预测脑泰方有效成分的作用靶点,利用TTD数据库检索出脑梗死涉及的靶点,通过生物学信息注释数据库(DAVID)预测分析相应靶点涉及的通路;然后对比不同途径获得的靶点及通路。结果脑泰方中有40个活性成分,预测得到靶点254个,预测到相关信号通路46条;疾病涉及的靶点26个,相关信号通路6条;其中药物活性成分预测的通路与疾病涉及的通路重合的有5条。结论通过数据对比分析可知,脑泰方干预脑梗死可能是通过调节补体和凝血级联通路、钙离子信号通路、肌动蛋白细胞骨架通路等多途径发挥作用,为进一步阐明脑泰方干预脑梗死具体的作用机制提供了重要参考。

Objective To explore the mechanism of action of Naotaifang in the treatment of cerebral infarction by identifying the main effective components of Naotaifang,predicting the targets and pathways through which the active components of Naotaifang act on cerebral infarction,and comparing the predicted targets and pathways with those related to cerebral infarction.Methods Taking advantage of the Traditional Chinese Medicine Systems Pharmacology(TCMSP)database and analysis platform,Traditional Chinese Medicine Database@Taiwan,and literature,the main effective components of Naotaifang were screened out.Based on the active components,targets of the effective components of Naotaifang were predicted with retrieval results from Pubchem database and Swiss Target Prediction database.The targets involved in cerebral infarction were retrieved from TTD database,and the pathways related to the targets were predicated and analyzed using the Database for Annotation,Visualization and Integrated Discovery(DAVID).Then,the targets and pathways obtained from different sources were compared.Results There were 254 targets and 46 related pathways predicated from 40 active components of Naotaifang.There were 26 targets and 6 related pathways involved in the disease.Five of the former pathways overlapped with the latter ones.Conclusion Through comparison and analysis of the data,it can be concluded that Naotaifang may act on cerebral infarction by regulating multiple pathways including the complement and coagulation cascade pathway,calcium signaling pathway,and actin cytoskeleton pathway,which provides an important reference for further elucidation of the mechanism of action of Naotaifang in the treatment of cerebral infarction.