超声不同指标评估肺高压大鼠肺动脉压力的实验研究

Experimental study of different ultrasonic modalities in evaluation of pulmonary arterial pressure in rats with pulmonary arterial hypertension

目的评价4种超声不同指标预测肺高压大鼠肺动脉压力的准确性。方法选取8周龄雄性大鼠41只,分为对照组11只和野百合碱组30只(MCT组),应用脉冲多普勒测定两组大鼠肺动脉血流加速时间(PAAT)、肺动脉射血时间(PET)及三尖瓣反流峰值流速(TR peak)。受试者工作特征(ROC)曲线分析PAAT、PAAT/PET、PAAT与心率比值(PAAT/HR)及TR peak预测大鼠肺动脉压力的诊断效能。结果两组大鼠PAAT、PAAT/PET、PAAT/HR及TR peak比较差异均有统计学意义(均P<0.05)。ROC曲线分析显示以PAAT/PET为0.381为截断值,其评估肺动脉压力的敏感性81.8%,特异性90.0%,准确率71.8%,明显高于PAAT和PAAT/HR的诊断效能。结论应用多普勒超声评估大鼠肺动脉压力时,PAAT/PET的预测准确性更高,可为无创诊断大鼠肺动脉高压提供新的手段。

Objective To evaluate the accuracy in estimating pulmonary arterial pressure(PAP)with four ultrasonic Doppler modalities in rats with pulmonary artery hypertension.Methods A total of 41 male rats at the age of 8 weeks were divided into control group(n=11)and monocrotaline group(MCT group,n=30).4 weeks after treatment,the pulmonary arterial acceleration time(PAAT),pulmonary arterial ejection time(PET)and tricuspid regurgitation peak flow velocity(TR peak)were measured by pulse Doppler.The diagnostic efficiency of PAAT,PAAT/PET,ratio of PAAT and heart rate(PAAT/HR)and TR peak in assessing PAP in rats were analyzed by ROC curve.Results There were significant differences of PAAT,PAAT/PET,PAAT/HR and TR peak between two groups(all P<0.05).ROC curve showed that PAAT/PET had the highest diagnostic value and its cut off value of 0.381 had 81.8%,90.0%and 71.8%in sensitivity,specificity and accuracy respectively on estimating PAP.Conclusion PAAT/PET is more reliable in assessing PAP in rats,which provides a new method for noninvasive diagnosis of pulmonary hypertension in rats.