Mda-7/IL-24和C-myb在弥漫大B细胞淋巴瘤中的表达及其临床意义

Clinical significance of Mda-7/IL-24 and C-myb expression in diffuse large B-cell lymphoma

目的:研究弥漫大B细胞淋巴瘤(DLBCL)中Mda-7/IL-24和C-myb的表达水平及其临床意义。方法:收集72例DLBCL患者的瘤组织及36例正常淋巴结组织;通过半定量逆转录PCR及免疫组化技术分别从mRNA及蛋白水平检测各组织中Mda-7/IL-24与Cmyb的表达水平,并对Mda-7/IL-24与C-myb进行相关性分析;收集患者临床资料,分析瘤组织Mda-7/IL-24与C-myb蛋白表达水平与患者年龄、性别、临床分期、是否骨髓浸润、Ecog评分及国际预后指数(IPI)之间的关系;分析瘤组织Mda-7/IL-24与C-myb蛋白表达水平与患者无进展生存期之间的关系。结果:与正常淋巴结组织相比,DLBCL组织中Mda-7/IL-24 mRNA的表达水平明显降低,而C-myb mRNA的表达水平则明显增高(P<0.01);72例DLBCL患者瘤组织中Mda-7/IL-24与C-myb mRNA表达水平呈明显负相关(r=-0.43,P<0.01);瘤组织低表达Mda-7/IL-24、高表达C-myb的患者其临床分期晚、易骨髓转移、瘤细胞增殖活性强、IPI积分较高,同时患者无进展生存期较短(P均<0.05)。结论:DLBCL组织中Mda-7/IL-24与C-myb的表达呈明显负相关,Mda-7/IL-24低表达或C-myb高表达是DLBCL患者临床预后较差的指标。

OBJECTIVE:This study aimed to investigate the clinical significance in expression of Mda-7/IL-24 and C-myb in diffuse large B-cell lymphoma(DLBCL)tissues.METHODS:Expression of Mda-7/IL-24 and C-myb in 72 tissues from DLBCL patients and 36 from normal lymphonodus tissues were assayed by reverse transcription polymerase chain reaction(RT-PCR)and immunohistochemistry.The relationship of Mda-7/IL-24 and C-myb expression and age,sex,clinical stage,bone marrow infiltration or not,Ecog scores,IPI index and survival was analyzed,and survival curves were prepared.RESULTS:The results show that compared with normal lymphonodus tissues,expression of Mda-7/IL-24 mRNA was higher,whereas expression of C-myb mRNA was lower.The expression of Mda-7/IL-24 mRNA was negatively correlated with C-myb(r=-0.43,P<0.01).Low expression of Mda-7/IL-24 or high expression of C-myb were positively correlated with malignant grade,bone morrow metastases,IPI scores and shorter survival period(all P<0.05).CONCLUSION:In summary,there was a negative correlation between Mda-7/IL-24 and C-myb expression in DLBCL tissues.The low expression of Mda-7/IL-24 and high expression of C-myb could be used as predictors for poor clinical conditions and prognosis of DLBCL.