补肾活血化痰开窍方对耳鸣大鼠耳蜗螺旋神经节神经元5-HT1B、2C受体表达的影响

The Effects of Bushen Huoxue Huatan Kaiqiao Decoction on the Expression of 5-HT1B,2C in Cochlear Spiral Ganglion Neurons in Tinnitus Rats

目的探讨补肾活血化痰开窍方对耳鸣大鼠耳蜗螺旋神经节神经元(spiral ganglion neurons,SGN)中5羟色胺(serotonin,5-HT)1B、2C受体的表达及意义。方法 60只健康SPF级雄性大鼠随机分为正常组10只(生理盐水组),耳鸣模型组50只(采用水杨酸钠腹腔注射联合"饮水抑制"大鼠行为学实验方法建立大鼠耳鸣模型),耳鸣模型组造模成功后再分为水杨酸钠组10只、中药组30只(补肾活血化痰开窍方低、中、高剂量组各10只)、西药组(卡马西平组)10只,除水杨酸钠组给予生理盐水灌胃外,各组给予相应药物干预8周,造模成功后运用免疫组化法检测各组耳蜗SGN中5-HT1B、2C受体的表达。结果耳鸣造模成功并给予相应药物干预8周后,各组大鼠耳蜗SGN中均有5-HT1B、2C受体的表达,与生理盐水组相比,水杨酸钠组中5-HT1B表达减少,5-HT2C表达明显增多;与水杨酸钠组相比,中药各剂量组耳蜗SGN中5-HT1B表达增多,2C表达均明显降低。结论耳鸣大鼠耳蜗SGN中的5-HT1B受体表达减少、5-HT2C表达增加,应用补肾活血化痰开窍方后5-HT1B表达增多而5H2C表达降低,产生了拮抗作用,该方可能应用于耳鸣的治疗。

Objective To investigate the expression of serotonin(5-HT)1B and 2C receptors in Spiral Ganglion Neurons(SGN)of tinnitus rats and to study the significance of Bushen Huoxue Huatan Kaiqiao Decoction.Methods The rats were injected intraperitoneally with sodium salicylate and"drinking water suppression"to establish rat model of tinnitus.The rats were randomly divided into the normal group 10(saline group),and the model group 50(sodium salicylate group).The model rats was further divided into TCM group(nourishing kidney and promoting phlegm resuscitation,low,medium and high dose group)of 10,the western medicine group(carbamazepine 5H-two benzene and nitrogen miscellaneous Zhuo-5-methyl n amine)Group)of 10.Each group was given corresponding intervention for 8 weeks.After the successful modeling,the expression of 5-HT1B and 2C in cochlear spiral ganglion neurons was detected by immunohistochemistry technique.Results After the tinnitus model was successfully given the corresponding drug intervention for 8 weeks,the expression of 5-HT1B and 2C in the rat cochlea SGN,compared with the saline group,the sodium salicylate group 5-HT1B decreased while 2C significantly increased.Compared with the saline group,the sodium salicylate group 5-HT1B decreased and 2C significantly increased.Conclusion The SGN 5-HT1B in the tinnitus rat cochlea decreased while 2C expression increased.The application of Bushen huoxue huatan kaiqiao decoction may be used for tinnitus treatment.