肉制品中沙门氏菌污染状况及血清分型分析

Contaminant Status and Serological Typing of Salmonella in Meat Products

沙门氏菌是重要的食源性致病菌之一,不同的沙门氏菌血清型对于人和动物的致病性不尽相同,了解肉制品中沙门氏菌污染情况并进行血清型研究对食品安全有着重要的意义。按照食品安全国家标准方法 GB4789.4-2010对采集的肉制品共75份进行沙门氏菌的污染情况进行检测,并采用传统的血清凝集法和全自动微生物基因指纹鉴定系统(Ribo Printer~?)对分离到的沙门氏菌进行血清分型鉴定。对分离纯化的沙门氏菌进行了血清分型,两种方法分析结果一致:检出的沙门氏菌有鼠伤寒沙门氏菌、斯坦利沙门氏菌、姆班达卡沙门氏菌和沃兴顿沙门氏菌,均是能引起食物中毒的沙门氏菌血清型。肉制品中存在沙门氏菌的污染,提醒相关部门做好监控,以预防食源性疾病的发生。

Salmonella is one of the most important foodborne pathogenic bacteria in the world.Different Salmonella serotypes have different pathogenicity for human and animals.It has great significance for food safety to investigate the Salmonella contamination and its serotype in meat products.The identification of Salmonella in totally 75 samples was carried out according to the national standard method of food safety(GB 4789.4-2010).Serological typing of the isolated Salmonella strains was done using both Diagnostic Sera for Salmonella and RiboPrinter?.Four Salmonella isolates strains could be identified to the same serovar using these two methods.S.Typhimurium,S.Stanley,S.Mbandaka and S.Worthington,which these four serotypes were reported causing foodborne disease outbreaks,were found in meat products in this study.Salmonella contamination existed in the meat products,and so relevant departments should strengthen the supervision for the prevention of foodborne diseases.