酵母粉对黑腹果蝇生长发育及产卵能力的影响

Effects of Yeast Culture Addition on Development and Oviposition of Drosophila melanogaster

为探索不同浓度的酵母粉(0、1.5%、3.0%)对黑腹果蝇生长发育及雌虫产卵能力的影响,分别对果蝇幼虫发育时间、白色预蛹重量以及雌虫产卵数进行测定。结果表明:利用不添加酵母的培养基饲养的果蝇在1龄幼虫阶段即发生死亡;随着培养基中酵母粉含量的增加,果蝇幼虫发育时间缩短,当酵母粉含量为1.5%时,幼虫发育时间与理论值接近,同时,雌虫产卵量显著提高(P<0.05);酵母浓度对蛹重无显著影响(P>0.05)。研究结果为实验室果蝇的正常饲养以及果蝇发育生物学的研究提供了理论基础。

In an attempt to investigate the effects of yeast culture addition on development and oviposition of Drosophila melanogaster(D.melanogaster),the developmental time and white pupal weight of D.melanogaster as well as the egg laying numbers of the females reared in the culture medium with addition of 0%,1.5%and 3.0%of yeast culture were determined,respectively,and were statistically assessed.The results showed that the D.melanogaster reared in the culture medium without yeast culture addition died in the 1st larval stage;the developmental time of the larvae was shortened with the elevation of yeast culture addition concentration.When the added concentration of yeast culture was 1.5%,the developmental time of the larvae was similar to the theoretical value,meanwhile,the egg laying numbers of the females were found significantly increased(P<0.05);added concentration of yeast culture had no significant effect on white pupal weight of D.melanogaster(P>0.05).Our results provide a theoretical foundation for the normal culture of D.melanogaster in the labs and for the researches on developmental biology of D.melanogaster.