合肥野生动物园灵长类动物隐孢子虫感染情况调查

Investigation on the Prevalence of Cryptosporidium Infection in Primates in Hefei Wildlife Park

[目的]调查合肥野生动物园灵长类动物隐孢子虫的感染情况。[方法]采用饱和蔗糖水漂浮法对环尾狐猴、赤猴和狒狒等6种灵长类动物粪便进行卵囊浓集,采用抗酸染色法对其卵囊染色后进行形态学观察。在形态学观察基础上,对上述疑似粪样中的卵囊进行DNA提取,并采用PCR技术扩增隐孢子虫卵囊壁蛋白基因(COWP),以1%琼脂糖凝胶电泳鉴定PCR扩增产物,并对该园内灵长类动物感染隐孢子虫情况作统计分析。[结果]经形态学鉴定,初步判定从该动物园灵长类动物粪便中获得的卵囊大小为4.66μm×5.18μm,与报道的隐孢子虫形态特征相一致;利用PCR技术扩增得到的目的基因大小为377 bp,与预期结果相一致。数据统计表明:合肥野生动物园灵长类动物隐孢子虫感染率为16.67%。[结论]合肥野生动物园灵长类动物存在隐孢子虫感染情况,具有感染人畜的潜在风险。

[Objective]To investigate the prevalence of Cryptosporidium infection in primates in Hefei Wildlife Park.[Method]The feces samples were collected from Lemur catta,Erythrocebus patas,Papio hamadryas and the other three kinds of primates reared in Hefei Wildlife Park,in which the presence of oocysts were screened by saturated sucrose solution floating method.The oocysts were stained by acid-fast staining method and were morphologically observed.Furthermore,the genomic DNA of oocysts recovered from the feces samples were extracted and the Cryptosporidium oocyst wall protein(COWP)gene were amplified using PCR assay.The PCR product were subsequently identified by 1%agarose gel electrophoresis.The incidence of Cryptosporidium infection in primates in this wildlife park was calculated.[Results]The morphological identification demonstrated that the size of the obtained oocysts was 4.66μm×5.18μm which was similar to the morphological characteristics of the Cryptosporidium oocysts reported previously;the size of the PCR product was 377 bp which was consistent with the expected size of the COWP fragment;the incidence of Cryptosporidium infection in primates in this wildlife park was 16.67%.[Conclusion]The prevalence of Cryptosporidium infection in primates in Hefei Wildlife Park was observed,indicating a potential zoonotic risk.