盐胁迫条件下杨树bZIP转录因子全基因组分析

Genome-wide analysis of the bZIP transcription factors in Populus in response to salt stress

[目的]杨树是三北地区重要的经济树种与绿化树种,然而其生长发育常受到干旱、盐碱等逆境胁迫的限制。为探明bZIP转录因子在杨树生长发育及抗逆胁迫过程中的分子生物学机制。[方法]本文利用生物信息学分析方法获得杨树bZIP转录因子214条,结合转录组测序数据,对其蛋白分子结构及盐胁迫条件下表达模式进行系统分析。[结果]根据其保守域氨基酸序列将214条杨树bZIP转录因子分为A、B、C、D、E、F、G、H、I、S及其他等11个亚族。盐胁迫处理后,14个杨树bZIP转录因子基因表达量显著上升,5个bZIP转录因子基因表达量显著下调,说明bZIP转录因子在杨树耐盐胁迫应答反应过程中发挥着重要的调节作用。[结论]该研究为以后该转录因子家族功能的深入研究利用提供理论基础。

[Objective]Populus is a genus of trees with economic importance and widely used in ornamental landscaping in north China.However,the growth and developmental rates of poplar trees are often impacted by drought,saline-alkali,and other adversity conditions.[Methods]To explore the underlying mechanism of bZIP transcription factors′effects on the growth,development,and stress tolerance ability of poplar trees,214 poplar bZIP transcription factor genes were obtained using bioinformatics methods in the present study.[Results]The protein structure and expression pattern of obtained genes under different stress conditions were analyzed by combining with RNA-sequencing data.The sequence alignment showed that 214 poplar bZIP transcription factors were divided into A,B,C,D,E,F,G,H,I,S,and another subgroup based on the amino acid sequence in the conserved domain.Under salinity stress condition,the expression level of 14 bZIP transcription factor genes was significantly up-regulated,and was down-regulated for another five genes.[Conclusion]The obtained results indicated that bZIP transcription factor may play an important role in plant salt tolerance regulation process,and it can provide a theoretical basis for further study of bZIP transcription factor.