响应面优化南极磷虾虾青素的复合酶法提取工艺研究

Optimization of multi-enzymatic extraction of astaxanthin from Antarctic krill via response surface methodology

为加大对南极磷虾Euphausia superba高附加值产品的开发,提高南极磷虾资源的综合利用率,以冷冻南极磷虾为原料,采用Alcalase碱性蛋白酶和木瓜蛋白酶复合酶解方法,以无水乙醇为提取剂提取虾青素,在单因素试验的基础上,以虾青素提取率为响应值,利用响应面优化设计,研究了虾青素的最佳提取工艺条件。结果表明:通过单因素试验筛选出酶底比、酶解时间和酶解温度为影响虾青素提取率的主要因素,优化得出最佳工艺条件为酶底比1.6%(Alcalase碱性蛋白酶与木瓜蛋白酶比例为3∶2)、酶解时间2.1h、酶解温度51℃、水料比(m L∶g)4∶1、无水乙醇料液比(g∶m L)1∶4、振荡时间10 min,在此优化条件下虾青素提取率为90.42%±0.39%,与模型理论值接近。研究表明,采用双酶复合酶解法能显著提高南极磷虾虾青素的提取效率,可为南极磷虾虾青素的制备提供技术支持。

Astaxanthin was extracted from Antarctic krill Euphausia superba via enzymatic hydrolysis by alkaline protease Alcalase and Papain using absolute ethanol as extractant,and the extraction conditions of astaxanthin were optimized by response surface design with astaxanthin extraction as the response value on the basis of single factor test in order to enhance the development of high value products of Antarctic krill,and to improve the comprehensive utilization of Antarctic krill resources.The results showed that the main factors affecting the extraction of astaxanthin included enzyme addition,enzymolysis time and enzymolysis temperature.The astaxanthin extraction rate of 90.42%±0.39%,which is close to the theoretical value of the model,was observed under the conditions of enzyme-substrate ratio of 1.6%(the ratio of alkaline protease Alcalase to Papain=3∶2),enzymolysis time 2.1 h,enzymolysis temperature 51℃,water-material ratio=4∶1(mL/g),material-ethanol ratio=1∶4(g/mL),and 10 min oscillation,indicating that double enzyme complex enzymatic hydrolysis method can significantly improve the extraction rate of astaxanthin in Antarctic krill,and that can provide technical support for preparation of astaxanthin in Antarctic krill.